Cambridge Scientific
Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Waterbaths, Circulators and Chillers ranging from Heated/Heating Water Baths to Refrigerated/ Refrigerating Cooling Water Baths, Shaking Water Bathsand Circulating Chillers to Recirculating Chillers. Some of our popular manufacturers include Fisher, VWR, Forma, Precision, Neslab and Brinkmann Lauda

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Life Technologies iBind Gel Electrophoresis

Life Technologies iBind Gel Electrophoresis

Cambridge Scientific ID #'s: 17623, 17624, 17355

Description:

The iBind™ is a benchtop device utilizing sequential lateral flow (SLF) to perform hands-free blocking, antibody binding, and washes for western detection workflows. The iBind™ uses no external power source, and relies on mechanical pressure from the iBind™ on a iBind™ Card to generate the sequential flow of immunodetection reagents for performing the blocking, antibody binding, and wash steps involved in western detection workflows

Specifications:

  • Dimensions (L x W x H): 24.2 x 14.6 x 3.5 cm
  • Material: Aluminum, plastic (PC/ABS), steel, silicone, neodymium (magnets)
  • Operating Temperature: 18ËšC to 30ËšC
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Life Technologies iBlot Dry Blot System

Life Technologies iBlot Dry Blot System

Cambridge ID #: 17202

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

  • Fast— protein transfer in 7 minutes or less
  • High quality transfer— efficient transfers without bubbles or distortions
  • Convenient—Self contained unit without the need for additional buffer or external power supply
  • Reproducible—reduces blot preparation and running variability
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Invitrogen iBlot Dry Blot System

Invitrogen iBlot Dry Blot System

Cambridge Scientific ID #'s: 16364, 13318, 19086, 11396, 13317, 15443, 19085, 13316, 18342, 15206, 19083, 13478, 14247

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

  • Fast— protein transfer in 7 minutes or less
  • High quality transfer— efficient transfers without bubbles or distortions
  • Convenient—Self contained unit without the need for additional buffer or external power supply
  • Reproducible—reduces blot preparation and running variability
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Life Technologies iBlot 2 Dry Blot System

Life Technologies iBlot 2 Dry Blot System

Cambridge Scientific ID #'s: 15207, 17640, 15208

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

  • Complete protein transfer in 7 minutes or less
  • High detection sensitivity and even transfer
  • Increased blotting reliability and reproducibility
  • Flexible gel size formats and membrane types
  • A simple, user-friendly system
  • Options to create new custom programs
  • Built-in tutorial and application notes
  • High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

  • For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
  • Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
  • Allows users to create custom programs

iBlot 2 Transfers Save Time

  • With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
  • The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

  • Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
  • The high density of ions in the gel matrix enables rapid protein transfer
  • During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
  • Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
  • Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

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Bio-Rad Chemidoc XRS Gel Imaging System

Bio-Rad Chemidoc XRS Gel Imaging System

Cambridge Scientific ID #'s: 14876, 17686, 16053, 18377

Includes:

  • Cabinet & Camera
  • Computer With Software
  • Manual

 

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

 

Specifications:

  • CCD Resolution (HxV): 1,392 x 1,040µm
  • Pixel density: 65,536 gray levels
  • Imaging area: 25 x 26 cm
  • Camera cooling temperature: -50°C
  • Camera cooling system: Peltier
  • Dynamic range: 4 orders of magnitude
  • Motorized zoom lens: C-mount, f/1.2, 12-75 mm
  • Illumination modes: Epi-white, trans-UV (302 nm), and white
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Invitrogen iBlot 2 Dry Blot System

Invitrogen iBlot 2 Dry Blot System

Cambridge ID #: 13321

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

  • Complete protein transfer in 7 minutes or less
  • High detection sensitivity and even transfer
  • Increased blotting reliability and reproducibility
  • Flexible gel size formats and membrane types
  • A simple, user-friendly system
  • Options to create new custom programs
  • Built-in tutorial and application notes
  • High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

  • For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
  • Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
  • Allows users to create custom programs

iBlot 2 Transfers Save Time

  • With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
  • The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

  • Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
  • The high density of ions in the gel matrix enables rapid protein transfer
  • During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
  • Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
  • Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Learn More
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Bio-Rad Trans-Blot SD Semi-Dry Transfer Cell

Bio-Rad Trans-Blot SD Semi-Dry Transfer Cell

Cambridge ID #: 10519

Features and Benefits

 

  • Transfers take place in as little as 15–60 min
  • Minimal buffer requirements save money
  • Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
  • Single-step locking system allows simple, speedy setup
  • Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
  • Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
  • Safety cover breaks the electrical current when lifted, preventing electrical shock

 

Applications and Uses

 

  • Western blotting
  • Nucleic acid transfer
  • Northern and Southern blot transfers in 10–35 min
  • Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

 

Features:

 

  • Maximum gel size (W x L), cm: 24 x 16
  • Buffer requirement, ml: 200
  • Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
  • Recommended power supply: PowerPac HC (High Current)
  • Dimensions (W x L x H), cm: 37 x 24 x 11
  • Weight, kg/lb: 3.6/7.9
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