Description
The Amersham Biosciences/GE AKTA Purifier 10 is a modular, automated Fast Protein Liquid Chromatography (FPLC) system designed for fast, reliable protein, peptide, and nucleic acid purification at microgram to milligram scales. Operating at flow rates up to 10 ml/min and pressures up to 25 MPa, it features automated buffer preparation and multi-wavelength UV-Vis monitoring via Unicorn software.
Key Components & Specifications
- System Components: Typically includes a P-900 pump, UPC-900 monitor, INV-907 valve, M-925 mixer, and Frac-920 fraction collector.
- Performance: Flow rates range from 0.001 to 10ml/min with a maximum pressure of 25 MPa (3626 psi).
- Monitoring: The UPC-900 enables monitoring of UV (up to 3 wavelengths), conductivity, and pH.
- Automation: Offers automated online buffer preparation (BufferPrep) and optimization for efficient purification.
- Software: Controlled by Unicorn software, allowing for method development and automated runs.
