Description
The Gel Doc EZ System is a compact and automated gel imaging instrument designed to yield publication-quality images and analyzed results with just the push of a button. The Gel Doc EZ System provides unparalleled flexibility with the use of four application-specific trays:
- UV tray for ethidium bromide staining of DNA gels and fluorescence imaging
- White tray for Coomassie, copper, silver, and zinc stains
- Blue tray for nucleic acid applications with SYBR stains
- Stain-free tray for stain-free imaging with Bio-Rad stain-free gels
The intuitive Image Lab Software bundled with the Gel Doc EZ Gel Imaging System includes capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy.
Features and Benefits of the Gel Doc EZ System
- Easy to use — researchers unfamiliar with the Gel Doc EZ imaging system can learn to use it quickly and easily because there is no need for manual control of filters, lens, or lights. Create a default protocol once, then use the green button on the front of the instrument to use these gel imaging and analysis settings time and again
- Publication-quality images and full data analysis — get clean and smooth publication-ready images with decreased pixelation when images are cropped or zoomed. Get analyzed results including relative molecular weights, bands quantitation, Excel reports, PDFs, and more in a matter of minutes
- Time and space savings — obtain results faster without spending time on various steps in image acquisition and save valuable bench space using this compact system
- Modular design and flexible options — use specific trays for different gel imaging applications. Clearly defined and color-coded trays eliminate confusion. Purchase only what you need and upgrade as your research needs change
- Stain-free technology — condense your 2 hr Coomassie protocol into a 5 min stain-and-image step with this stain-free gel imaging system. Bio-Rad stain-free gels are western blot compatible, allowing you to check electrophoresis results and quality prior to western blotting. These “green” gels provide equal or better sensitivity than Coomassie staining and eliminate organic waste disposal problems
Specifications
Applications:
- Chemiluminescence: No
- Fluorescence*: Yes (stain-free imaging* and UV-excitable dyes)
*requires Bio-Rad stain-free gels
- Colorimetry/densitometry: Yes
- Gel documentation: Yes (Flamingo™, Oriole™, SYPRO Ruby, Coomassie, Fluorescent Orange, or Krypton Stains; ethidium bromide, GelGreen, Gel Red, SYBR® Safe, SYBR® green, or SYBR® Gold dyes; Bio-Rad stain-free gels)
Hardware Specifications
- Maximum sample size: 11.2 x 15 cm (4.4 x 6 in)
- Maximum image area: 11.2 x 15 cm (4.4 x 6 in)
- Excitation source: Trans UVB (302 nm)
Illumination control
- 2 modes: Trans-UV, Trans-White
- UV tray: 280-400 max 300 nm
- Blue tray: 430-460, max 440 nm
- White tray: visible spectra
- Stain-free tray: requires Bio-Rad stain-free gels
- Detector: CCD
- Image resolution: 1392 x 1040 pixels (1.4megapixels)
- Emission filters: Tray-based
- Dynamic range: >3.0 orders of magnitude
- Pixel density (gray levels): 4,096
- Dynamic flat fielding: Yes
- Instrument size (L x W x H): 43 x 28 x 38 cm (17 x 11 x 15 in)
- Instrument weight: 7.3 kg (16 lb)
Operating Ranges
- Operating voltage: 110/115/230/240 V AC nominal
- Operating temperature: 10–28°C (21°C recommended)
- Operating humidity: <70% non condensing
Automation Capabilities
- Workflow automated selection: Application-driven; user-selected or recalled by a protocol
- Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
- Workflow reproducibility: 100% repeatability via recallable protocols, from image capture to quantitative analysis and reports
- Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
- Image flat fielding**: Dynamic; precalibrated and optimized per application
- **U. S. patent 5,951,838
- Auto-exposure: 2 user-defined modes (intense or faint bands)
