Description
The Octet QKe system is the enhanced-performance version of the first-generation Octet QK instrument from the Octet family of label-free biomolecular interaction analysis instruments. Two acquisition rate settings and a higher-performance spectrometer combine to provide greater sensitivity and a wider dynamic range for protein quantitation and kinetic analysis. In addition, biosensor re-racking provides enhanced assay flexibility and operational cost savings.
- Detection Technology: Bio-Layer Interferometry (BLI)
- Biosensor Type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking
- Information Provided: Kinetic and affinity analysis (kobs, ka, kd, KD); Concentration data analysis (no need for background subtraction); Epitope binning and cross-blocking matrices and trace overlays
- Data Presentation: Plots displaying kinetic binding, equation fits, and residuals of fits; Tabulated kinetic and quantitation data
- Sample Types: Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
- Sample Plate: Standard 96-well, black, flat bottom microplate
- Sample Volume: 180–220 μL/well (96-well microplate); Nondestructive testing, easily recovered
- Orbital Flow Capacity: Static or 100–1500 rpm
- Analysis Temperature Range: (Ambient + 4°C)–40°C, 1°C increments
- Throughput: Up to 8 assays in parallel; up to 96 assays per 96‑well microplate
- Analysis time per sample: hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples; Real-time kinetic analysis experiments from 5 minutes to 4 hours
- Baseline noise: – ≤ 3 pm (RMS) at High Sensitivity acquisition rate; – ≤ 8 pm (RMS) at Standard acquisition rate
- Quantitation Range for hlgG: 1 µg/mL to 700 µg/mL at low rpm LOD of 0.05 µg/mL at 1000 rpm
- Dimensions: 18.6 in (H) x 17 in (D) x 20.8 in (W) [47 cm (H) x 43 cm (D) x 53 cm (W)]
- Weight: 54 lb (24.5 kg)
- Electrical requirements: – Mains: AC 100–240 V, 5.0–2.0 A, 50/60 Hz, single phase; Power consumption: 120 W (240 W peak)
- Safety standards: CE, Nemko
