Description
The SpectraMax iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
Specification:
- Read modes:
- Absorbance
- Fluorescence (top/bottom read),
- Luminescence (top read)
- Wavelength ranges:
- Abs: 230 – 1000 nm
- FL Ex: 250 – 830 nm
- FL Em: 270 – 850 nm
- Lumi: 300 – 850 nm
- Wavelength selection: Monochromator tunable in 1-nm increments
- Absorbance photometric accuracy/linearity: < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
- Absorbance photometric precision/repeatability: < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
- Sensitivity top read optimized:
- FL:1 pm fluorescence 96 well
- 1 pm fluorescein 384 well
- Lumi: 2 pm ATP 96 well, 4 pm
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- Light source(s): High power xenon flash lamp
- Detector(s): Silicon photodiode, Ultra-cooled photomultiplier tube
- Read types (endpoint, kinetic, etc): Endpoint, Kinetic, Spectrum scan, Well scan
- Read times:
- Abs: 28 seconds,
- FL: 21 seconds
- Lumi: 21 seconds
- Plate shaking: Linear, Orbital, Double orbital
- Plate type(s): 6-384 well plates
- Temperature control: Ambient + 5°C to 66°C
- Dimensions: H 40.1 cm x W 53.2 cm x D 59.8 cm
- Validation tools:
- Absorbance validation plate
- Fluorescence validation plate
- Luminescence validation plate
- SoftMax® Pro GxP Software
