Thermo Scientific Nanodrop 2000 Spectrophotometer UV/Vis Reader

Cambridge Scientific ID: 28644 Category: Tag:



Enhance the limit of detection for DNA, RNA and protein with the Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer featuring both microvolume pedestal and cuvette measurement options. The cuvette station is ideal for measuring dilute nucleic acid and protein assays, includes heating and microstirring capabilities, and allows users to perform kinetics and cell culture readings (600nm).

Wide spectral range (190-840nm) for measuring a variety of samples types:
Peptides (205nm)
DNA and RNA (260nm)
Purified protein (280nm)
Toxicology assays and industrial dyes (490nm)
Gold nanoparticles (520nm)
Colorimetric protein assays (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
Optical Density measurements (600nm)
Combining the patented sample retention system with cuvette capability accommodates low and high concentrations
(0.4 to 15,000 ng/µL dsDNA and 0.01 – 400 mg/mL BSA)
Pedestal measurements that require only 0.5 – 2 µL of your sample and do not require sample dilution, even for highly concentrated samples
Calculates sample purity ratios (260/280 nm and 260/230 nm)
Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
Fast measurement time of less than 5 seconds, 10 -15 seconds from sample pipette to wipe
User-friendly software that includes custom methods and data export capabilities
Nanodrop 2000C Specifications:

Instrument Type: Spectrophotometer
Minimum Sample Volume: 0.5 µl
Recommended Sample Volume, Nucleic acid: 1 µl
Recommended Sample Volume, Proteins: 2 µl
Sample Number: 1
Path Length: 1 mm (auto-ranging to 0.05 mm)
Light Source(s): Xenon flash lamp
Detector Type: 2048 – element linear silicon CCD array
Wavelength Range: 190 – 840 nm
Wavelength Accuracy: 1 nm
Spectral Resolution: ≤ 1.8 nm (FWHM at Hg 253.7 nm)
Absorbance Precision: 0.002 (1 mm path)
Absorbance Accuracy: 3% (at 0.74 at 350 nm)
Absorbance Range: 0.04 – 300 (10 mm equivalent)
Detection Limit: 2 ng/µl (dsDNA)
Maximum Concentration: 15,000 ng/µl (dsDNA)
Measurement Time: 5s (typical)
Footprint: 14 x 20 cm
Weight: 2.1 kg
Sample Pedestal Material of Construction: 303 stainless steel and quartz fiber
Operating Voltage: 12 vdc
Operating Power Consumption: 12 – 18 W (max 30 W)
Standby Power Consumption: 5 W
Software Compatibility: Operating software for the NanoDrop 2000 and NanoDrop 2000c. Compatible with Professional versions of Windows 8.1 (64 bit), and Windows 7 (32 and 64 bit).
Cuvette Specifications:

Z-Height: 8.5 mm
Heating: 37 ± 0.5 °C
Stirrer: 150-850 rpm
Path Length: 10, 5, 2, 1 mm
Absorbance Range: 0.008 – 1.5
Detection Limit: 0.4 ng/µl (dsDNA)
Maximum Concentration: 750 ng/µl (dsDNA)
Measurement Time: < 3 seconds Cuvette Dimensions: 12.5 mm x 12.5 mm, up to 48 mm H Type: Masked cuvette UL/CSA and CE: All units are approved to these standards

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