Description
The Waters Acquity UPLC (Ultra Performance Liquid Chromatography) System is a modular chromatography platform used for high-resolution separation, identification, and quantification of compounds in complex samples. Paired with the Acquity UPLC PDA eλ (Photodiode Array Extended Lambda) Detector, the system enables full-spectrum UV/Visible detection across an extended wavelength range, allowing researchers to capture absorbance data in both the ultraviolet and visible regions simultaneously. A typical system configuration includes the PDA eλ Detector, Binary Solvent Manager, Sample Manager, and a column heater, along with a computer preloaded with control software. The system is widely used in pharmaceutical, environmental, and analytical laboratories for method development, compound identification, trace impurity detection, and routine quality control analysis.
Features:
- Ultra Performance Liquid Chromatography (UPLC) technology: Uses small-particle columns and high system pressure to achieve reduced dilution and band broadening compared to conventional HPLC, maintaining sharper peak shape, height, and sensitivity, resulting in faster run times and higher resolution separations.
- Extended-wavelength Photodiode Array (PDA eλ) detection: Provides high optical and digital resolution with low signal-to-noise ratios and library matching, allowing quantitation of trace impurities and simultaneous 2D and 3D spectral operation across both UV and visible regions, so analysts can identify and confirm compounds by their full spectrum rather than a single wavelength.
- Low-volume, light-guiding flow cell: Improves light transmission efficiency and allows quantitation up to 2.0 AU without compromising linearity, which is important for matching detector performance to the small peak volumes produced by UPLC columns.
- Extended wavelength range: Covers 190–800 nm, extending sensitivity into the visible spectrum for a broader range of compounds, including colored or visible-light-active analytes, beyond what a standard UV-only PDA detector can capture.
- Software integration: Fully compatible with Waters’ Empower and MassLynx chromatography data systems, supporting method development, data acquisition, and regulatory-compliant reporting.
- Modular system design: Built around interchangeable modules (solvent manager, sample manager, detector, column heater), allowing labs to configure and upgrade the system to match specific throughput, sensitivity, or application needs.
Specifications:
- Wavelength Range: 190–800 nm
- Optical Resolution: 1.2 nm
- Photodiode Array: 512 photodiodes
- Linear Range: Up to 2.0 AU without compromising linearity
- Flow Cell: Low-volume, light-guiding (Teflon AF) design
- Compatible Software: Empower, MassLynx
- Typical System Modules: Binary Solvent Manager, Sample Manager, PDA eλ Detector, Column Heater
