Description
The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.
Specifications:
Measurements:
- Molar Mass Range: 200 Da to 1 GDa 1
- Molecular Size Range (R g ): ≈ 10 to 500 nm, up to 1000 nm with shape-specific models
- Molecular Size Range (R h ): In flow mode, 0.5 nm to 300 nm 2 ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection).
- Sensitivity: 10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter
Fluidics:
Detectors;
- MALS Detectors: High-gain, high dynamic range photodiodes at 18 detection angles.
- Auxiliary Detectors: Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.
- Dynamic Range: 3,300,000:1
- DLS Detector (optional): WyattQELS dynamic light scattering module installs directly inside the DAWN chassis. Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument or Mobius DLS-ELS instrument may be installed in the DAWN.
Laser Properties:
- Laser Wavelength: 658 nm ± 4 nm; 785 nm optional for use with fluorescing samples
- Laser Power Control: Programmable 10% to 100%
Flow Cells: Fused Silica, optimal for solvent refractive index less than 1.50; F2, optimal for solvent refractive index above 1.50
Temperature Options:
- Ambient
- Heated/Cooled (HC) -15 °C to +150 °C
- Ultra-High-Temperature (UHT) ambient to +210 °C
Electronics:
- Analog Inputs: 4 differential analog inputs with 24 bit resolution. Input range -10 V to +10 V
- Analog Outputs: 2 analog outputs from user selectable measurements channels -10 V to +10 V
- Other Inputs/Outputs: Alarm in, Alarm out/retransmit, Auto-Inject in, Auto inject contact closure retransmit, Recycle In/Out (operates Orbit ™ recycle valve)
- Computer Interface: Ethernet
- Front Panel Display: 10.1″ (257 mm), capacitive high-resolution screen with multi-touch functionality, displays signals, graphs, instrument settings and diagnostics
Dimensions: 58 cm (L) x 36 cm (W) x 18 cnm (H)
