Used Lab Equipment

Waters Acquity/TQD system from 2012 including:

• ACQUITY I-Class BSM, binary solvent manager
• ACQUITY I-Class SM-FL, sample manager
• ACQUITY H-Class Col Mgr-Active, column manager with 2 column positions
• ACQUITY PDA DETECTOR, uv detector
• XEVO TQD, mass spec detector
• Computer loaded with Mass Lynx/Empower software
• Roughing pump and UPS backup

Includes:

• Qty (2) - G1361A Agilent 1100 Series Prep Pumps
• G1315A DAD Diode Array Detector (G1314A VWD Detector also available)
• G2258A Agilent 1100 Prep Dual Loop Autosampler (Manual Injector also an option)
• G1364B AFC Automated Fraction Collector
• Computer Loaded with ChemStation
• Monitor – Mouse – Keyboard
• Cables and Manuals

G1361 Prep Pump

Features:

• Extended flow rate range
• Without changing pump heads, provides flow rates up to 100 mL/min at 400 bar
• For preparative and analytical applications with low flow rates (0.5 mL/min, upward), provides precise high-pressure binary gradient formation with low delay volume (700 uL)
• Automated seal wash using a peristaltic pump extends seal lifetime
• Pumps stop in response to column blockage or leaks
• Automated electromagnetic purge valve simplifies system setup
• Software provides solvent level control that keeps the pump from running dry

A proprietary band function enables obtaining accurate fractions even when the peak elution time varies, by catching the target component according to variations in the chromatogram. If connected to workstation software, fractions can be simulated on screen to determine whether or not the fraction program is appropriate.

Details:

• Small and Large Volume Collection Capacity
• The FRC-10A collects both analytical and preparative fractions. The standard configuration has a 144 5mL vial capacity. The large volume kit can be added for large volume collection.
• Precise Peltier Temperature Control
• For automated collection of thermally-labile fractions, Peltier temperature control keeps temperatures stable within a 4-70º C range.
• Simplified Graphical Parameter Entry
• Set up fractionation parameters graphically from the SCL-10AVP System Controller. Eliminate the guesswork of determining slope and level values.
• Fraction Collection Flexibility
• Tailor the collection to your application requirements.  Choose from collection criteria such as volume, threshold, and peak purity. A precise peak division feature ensures high purity fractions. The FRC-10A automatically adjusts for retention time shifts, so that only the peak of interest is collected. Our slope-override capability enables continued collection, even when the detector signal is off-scale.

Specifications:

• Drive System and Fraction Collection Methods: Arm-Movement X-Y System
• Select either solenoid valve or direct through nozzle
• Fraction Modes: Manual or programmed fraction collection by combining 14 types of parameters, such as slope, level, and band
• Number of Fractions: 16 to 144
• Collection Tubes: Glass: 3.5, 5, 20, 32, or 50 mL, or user supplied
• Polypropylene: 4.5, 5, 25, or 50 mL, or user supplied

Description:

System controller equipped with a data buffering function and acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet. Up to eight units can be connected to the box-type CBM-20A. The card-type CBM-20Alite is used by incorporating it into an LC-20A-Series solvent delivery pump or auto-sampler. It can control up to five units, including the unit in which it is incorporated.

Specifications:

Connectable Units:

Solvent delivery units: 4 max
Autosamplers: 1
Column ovens: 1
Detectors: 2 max
Fraction collectors: 1
Sub-controllers: 2 max."
Number of connectable units: 8 (expansion possible up to 12)
Data Buffering: Approx. 24 hours for one analysis (at 500 ms sampling rate; available only with LCsolution)
Event I/O: 4 inputs, 4 outputs
Analog Boards: Up to 2 boards can be mounted
Operating temperature range: 4°C to 35°C
Dimensions, weight: 260 (W) x 140 (H) x 420 (D) mm, 5.5 kg
Power requirements: 100 VAC, 100VA, 50/60HZ

Features:

• <400 uL internal volume
• Plugs into LC-20A/LC-30A series pump for power and control
• New material (Systec AF) allows high gas permeability
• Peak efficiency in under 1 minute
• Additional channel provided for autosampler rinse solution

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Includes:

• AB Sciex API4000 LC/MS/MS
• Enhanced high performance triple quadrupole mass spectrometer system
• Line transformers
• Turbo V Ion Source (with HESI probes)
• Roughing Pump
• Windows 7 Computer loaded w/ Analyst Software 1.6
• Cart
• Optional Shimadzu Front End
• Monitor – Mouse – Keyboard
• Cables and Manuals

Specifications:

• Detector type: Pulse Counting CEM
• Dynamic Range: 5 orders of magnitude
• Ionization Sources: ESI, APCI
• Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
• Mass Range: 5-3,000
• Polarity Switching Time: 700 ms
• Scan Speed (Quad): 24,000 Da/sec
• Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
• Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%

Optional Shimadzu UFLC System Components:

• SIL-20AC
• LC-20AB
• CTO-20AC
• CBM-20A
• DGU-20A₅

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1200 Series Dual Loop Autosampler is a fixed loop sampler with two different sample loops. As a consequence of this design the metering device, also referred to as syringe, is not a part of the solvent flow path from pump to HPLC column. An additional flush solvent bottle is used to rinse this part of the hydraulic path after each injection. In order to minimize carryover, the sampler is equipped with a needle wash function which dips the needle into a wash vial or the wash port. The wash port is fed by a peristaltic pump and can be operated with most common HPLC solvents.

Specification:

• Weight: 14 kg (29.8 lbs)
• Dimensions (H x W x D): 200 x 345 x 440 mm (8 x 13.5 x 17 in.)
• Line voltage: 100 - 240 VAC, ±10 % (wide-ranging capability)
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 200 VA
• Power consumption (active power): 180 W
• Ambient operating temperature:  4 - 55 °C (41 - 131 °F)
• Ambient non-operating temperature: -40 - 70 °C (-4 - 158 °F)
• Humidity: < 95 %, at 25 – 40 °C (77 – 104 °F) non condensing
• Operating altitude: up to 2000 meters (6500 ft)
• Non-operating temperature: top to 4600 meters (14950 ft)
• Safety standards (IEC, CSA, UL): Installation category II, pollution degree 2

Description:

The Parker NitroFlow TG2 is a self-contained gas generator that produces up to 80 lpm of pure LCMS grade gases. Designed for the AB Sciex 6500 series, zero air is produced at pressure above 110 psig for Gas1 and Gas2 needs. This combination of Parker technologies yield the highest performing, most reliable and most quiet integrated TriGas generation system available.

Specifications:

• Sound Level: <49 dB(A)
• Min/Max Ambient Temperature: 60°F/85°F (16°C/29°C)
• Electrical Requirements: 208-230 VAC, 60 Hz, 1 Phase, 16A
• Dimensions (H x W x D):  43” x 21” x 34” (109 x 53 x 86 cm)
• Weight 427 lbs. (194 kg)
• Hydrocarbon Free
• Phthalate Free

Features:

• Extended flow rate range
• Without changing pump heads, provides flow rates up to 100 mL/min at 400 bar
• For preparative and analytical applications with low flow rates (0.5 mL/min, upward), provides precise high-pressure binary gradient formation with low delay volume (700 uL)
• Automated seal wash using a peristaltic pump extends seal lifetime
• Pumps stop in response to column blockage or leaks
• Automated electromagnetic purge valve simplifies system setup
• Software provides solvent level control that keeps the pump from running dry

Can be sold as an individual component or as part of a complete stack

For Preparative Scale Applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
  • Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
  • Diverter valve: ~15
  • Diverter valve to needle: ~110
  • Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

Specifications:

• Pressure: Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:
  • 900 µl in 0.1 µl increments (recommended 1 µl increments)
  • Up to 1800 µl with multiple draw (hardware modification required)
  • Up to 5000 µl with multiple draw (hardware modification required)
• Replicate injections: – 99 from one vial
• Precision:
  • Typically < 0.5% RSD of peak areas from 5 – 2000 µl,
  • Typically < 1% RSD of peak areas from 2000 – 5000 µl
  • Typically < 3% RSD of peak areas from 1 – 5 µl
• Minimum sample volume:
  • µl from 5 µl sample in 100 µl microvial
  • 1 µl from 10 µl sample in 300 µl microvial
• Sample viscosity range: 0.2 – 50 cp
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s, depending on draw speed and injection volume