Used Lab Equipment

Includes 1 year Service Contract, Installation, and a PM at 6 months

Optional TUV or PDA Detector

Developed for integrated UPLC/MS/MS quantitative applications, the Waters ACQUITY TQD features the highest levels of tandem quadrupole MS selectivity, robustness, speed, and accuracy.   To maximize the analytical advantages of UltraPerformance LC (UPLC), the ACQUITY TQD operates at the highest data acquisition rates. When integrated with the ACQUITY UPLC System, the ACQUITY TQD gives you class-leading analytical detection limits, resolution, and sample throughput for all of your quantitative applications: bioanalysis, ADME screening, food safety, environmental monitoring, clinical, forensic, and more.

Includes:

• Waters TQD UPLC/MS/MS Mass Spectrometer
• Enhanced high performance triple quadrupole mass spectrometer system
• Roughing Pump
• Windows 7 Computer loaded w/ Mass Lynx 4.1 SCN 805.IVD
• Flex Cart for UPLC
• Waters Acquity UPLC
• Column Manager
• Sample Manager
• Binary Solvent Manager
• Monitor – Mouse – Keyboard
• Cables and Manuals

Performance Specifications:

• Acquisition modes
  • Full scan MS
  • Product ion scan
  • Precursor ion scan
  • Constant neutral loss
  • Selected Ion Recording (SIR)
  • Multiple Reaction Monitoring (MRM)
• Survey scan modes: Full scan MS triggered product ion scan
• Mass range: 2 to 2048 m/z
• Scan speed: Up to 10,000 Da/s
• Examples of achievable acquisition rates:
  • 10 scans per second (m/z 100 to 1000)
  • 20 scans per second (m/z 50 to 500)
• Mass stability: Mass drift is < 0.1 Da over a 24 hour period
• Linearity of response: The linearity of response relative to sample concentration for a specified compound is five orders of magnitude from the limit of detection
• Polarity switching time: 20 ms to switch between positive and negat ive ion modes
• MRM acquisition rate: Minimum dwell time of 3 ms per MRM channel
• Inter-channel cross talk: The inter-channel cross talk between two MRM transitions, acquired using an MRM dwell time of 10 ms and an inter-channel delay time of 10 ms, will be less than 0.02%.
• Number of MRM channels: Up to 16,384 MRM channels (512 functions, 32 channels per function) can be monitored in a single acquisition; up to 1024 MRM channels when operating in GLP/secure mode (32 functions, 32 channels per function).
• Mass resolution: Automatically adjusted (InteUiStart) to desired resolu tion; The vaUey between the 2034.63 Da and 2035.63 Da peaks is < 12% of the average height of the two peaks
• MRM sensitivity (ESI+) : A 1 pg on-column injection of reserpine will give a chromatographic signal-ta-noise greater than 2,000:1 (Gradient separation, LC mobile phase flow rate of 0.8 mL/min, MRM transition mil 609 > 195).
• MRM sensitivity (ESI-): A five pg loop injection of chloramphenicol, wi th a mobile phase flow rate of 200 ~lImin will give a chromatographic signal-ta-noise for the transition 321 > 152 mlz greater than 180: 1.
• MRM sensitivity (APCI+): A 100 pg loop injection of 17-a-hydroxyprogesterone, with a mobile phase flow rate of 1000 IJLlmin will give a chromatographic signal-ta-noise for the transition 331 > 109 mil > 150:1.

Includes Installation and One Year Warranty (Preventative Maintenance at 6 months)

Method Development and Training also Available

Includes:

• AB Sciex API 3200 Mass Spectrometer LC/MS/MS
• Windows 7 Computer loaded w/ Analyst Software 1.5
• Turbo V-Spray ESI
• Vacuum pump
• Shimadzu Prominence 20 UFLC  Shimadzu
• Shimadzu DGU-20A3 Degasser
• (2)Shimadzu LC_20AD Liquid Chromatograph
• Shimadzu CTO Column Oven
• Shimadzu CBM-20A Communication Module
• CTC Autosampler
• Monitor – Mouse – Keyboard
• Manuals

The API 3200 LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package

• Innovative Turbo V ion source efficiently ionizes compounds over a wide range of flow rates, virtually eliminating cross-contamination, even with large sample loads
• Patented LINAC collision cell technology delivers superior MS/MS performance allowing faster scan times without compromising sensitivity or mass spectral quality
• Simplifies cross-platform methods transfer
• Gives meaningful results from every experiment

Specifications:

• Detector type: Pulse Counting CEM
• Dynamic Range: 5 orders of magnitude
• Ionization Sources: TurboIonSpray, APCI, DuoSpray, PhotoSpray
• Mass Accuracy / Stability: 0.1 Da over 8 hours and 0.15 Da over 24 hours
• Mass Range: 5-1,700 (Quad); 50-1,700 (LIT)
• Polarity Switching Time: 700 ms
• Scan Speed (Quad): 24,000 Da/sec
• Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, TripleTrap

Includes One Year Service Contract, PM at 6 Months, and Installation

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

Includes:

• AB Sciex 5500 Qtrap
• Shimadzu Prominence 20 UFLC  Shimadzu
  • Shimadzu DGU-20A3 Degasser
  • (2)Shimadzu LC_20AD Liquid Chromatograph
  • Shimadzu CTO Column Oven
  • Shimadzu CBM-20A Communication Module
  • CTC Autosampler
  • Computer Loaded with Analyst 1.6 Software       

Specifications

• Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
• V.: < 5%
• Triple quad scan speed: 12,000 Da/sec
• Linear ion trap scan speed: 20,000 Da/sec
• Polarity switching time: 50 msec
• Minimum MRM Dwell Time: 1 msec
• Triple quad mass range (m/z): 5-1250
• Linear ion trap mass range (m/z): 50-1000
• Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap™ scan modes

The Agilent 1290 Infinity Flexible Cube is an auxiliary module for the 1290 Infinity Autosampler with a built-in pump to facilitate enhanced flushing capabilities and to reduce carry-over. With access to three solvents, critical sections of flow path can be flushed at high flow rates. Automated back-flushing of the injection needle-seat achieves the absolute lowest sample carryover for LC and LC/MS applications. In addition, the Flexible Cube hosts up to two Agilent Quick-Change valves. For utmost flexibility, choose among Agilent’s broad portfolio of available valve heads suitable up to 1200 bar. The valves can be controlled independently for any demanding valve-switching application.

Specifications

• Injection range                          
  • 1 - 20 μL
  • .1 - 40 μL
  • .1 - 100 μL
  • in 0.1 μL increments
• Precision: Precision of G4226A sampler will not be influenced
• Accuracy: Accuracy of G4226A sampler will not be influenced
• Sampler pressure range: Up to 1200 bar
• Pressure range of flush pump: Up to 50 bar
• Flow rate of flush pump: Up to 4 mL/min
• Number of solvents: Up to 3 solvents
• Sample viscosity range: 0.2 – 5 cp
• Carry over:  Typically <0.001% (10 ppm)

Includes 1 year Service Contract, Installation, and a PM at 6 months

Includes:

• AB Sciex API4000 LC/MS/MS
• Enhanced high performance triple quadrupole mass spectrometer system
• Line transformers
• Turbo V Ion Source (with APCI and ESI probes)
• Roughing Pump
• Windows 7 Computer loaded w/ Analyst Software 1.6
• Cart
• Agilent 1100 HPLC and CTC Autosampler
  • G1322 Degasser
  • G1312A Binary Pump
  • G1316A Column Compartment
  • G1323A Game Boy
  • CTC Autosampler

• Monitor – Mouse – Keyboard
• Cables and Manuals

Specifications:

• Detector type: Pulse Counting CEM
• Dynamic Range: 5 orders of magnitude
• Ionization Sources: ESI, APCI
• Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
• Mass Range: 5-3,000
• Polarity Switching Time: 700 ms
• Scan Speed (Quad): 24,000 Da/sec
• Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
• Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%

The HP / Agilent 1100 HPLC series is the current model and arguably the best design in the industry because of the optimized design of the quaternary pump ensuring virtually pulse-free and stable solvent flow, with dual floating pistons in series, precisely servo-controlled. This design with variable stroke volume allows pulse-free solvent delivery and efficient mixing. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation with volatile solvents and is maintenance free. With high-speed proportioning valve and pre-configured vacuum degasser, for convenient low-pressure mixing of up to four solvents and highest flexibility in solvent choices.

 Features:

• Ease-of-use - handheld control module lets you enter operating parameters fast, store methods easily and display pressure profile, diagnostics and task-oriented information.
• Durable - stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE ensure long lifetime. Seal wash option for high salt mobile phases keeps maintenance to a minimum for lower operation costs.
• Wide flow range - up to 10 ml/min for broad HPLC work, including semi-preparative separations.
• Better for GLP - built-in leak and pressure test with graphical presentation of pressure profiles, and early maintenance feedback.

Specifications:

• Hydraulic system: Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve
• Settable flow range: .001– 10 ml/min, in 0.001 ml/min increments
• Flow range: 2 – 10.0 ml/min
• Recommended pH range: – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel.
• Operating voltage: 230V

Optional Column Selection Valve

Specifications:

• Temperature Range: 10° below ambient to 80° C
• Temperature Stability: ± 0.15°C
• Temperature Accuracy: ± 0.8°C
• Column Capacity: Three 30cm columns
• Warm-up/Cool Down: 5 mins from ambient to 40°C

Specifications:

• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications Controller: area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features:  Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:  
  • 1 – 100 μl in 0.1 μl increments
  • Up to 1500 μl with multiple draw (hardware modification required)
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 100 μl,
  • Typically < 1 % RSD from 1 – 5 μl
• Sample viscosity range:  0.2 – 5 cp
• Sample capacity:
  • 2 × well-plates (MTP) + 10 × 2 ml vials
  • 100x2ml in one tray
  • 40 x 2 ml in half tray
• Injection cycle time:
  • Typically < 30 s using following standard conditions:
  • Default draw speed: 200 μl/min
  • Default eject speed: 200 μl/min
  • Injection volume: 5 μl
• Carry-over Typically:
  • < 0.01 % using the following conditions:
  • Column: 125 x 4 mm Hypersil ODS, 5 μm
  • Mobile phase: Water/Acetonitrile = 80/20
  • Flow rate: 1 ml/min
  • Injection volume: 1 μl caffeine (1 mg/ml), 5 μl water to test carryover
  • Outside wash of needle before injection: 20 sec with water using flush port

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Injection range: From 0.1 – 100 µl in 0.1 µl increments
• Replicate injections: From 1 – 99 from one vial
• Injection cycle time: Typically 50 s, depending on draw speed and volume
• Sample capacity: 100 × 2 ml vials in tray

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

Features:

Install any desired configuration of Agilent 1200 Series modules. The control module software will reflect which modules are present in the LC system and adjust the screens accordingly.

• Enter parameter settings for every module, perform reset and on/off functions as well as calibration and configuration settings in a self-explanatory and intuitive way.
• Define automated analyses including methods, timetables, injector programs, method sequences and automated calibration settings using the control module.
• Protect your method from any inadvertent keyboard changes by setting method protection.
• Use PC cards to store and transfer methods and sequences between Agilent1200 systems.
• Monitor all operations and error events using the self-updating logbooks.
• Use the context-sensitive online information system to get further information on all topics.
• Use the context-sensitive menu function to have the quickest access on related functions.
• To help comply with Good Laboratory Practice (GLP) regulations you can select a variety of module tests that will check the performance of the LC system.
• The early maintenance feedback (EMF) limits can be used for scheduling maintenance work.
• Display data graphically using the Plot screen where as many as three different signal scan be monitored at the same time.
• Print information to a PCL3 compatible printer connected to the serial RS232 port of an Agilent 1200 module.