Used Lab Equipment

The Pharmacia Electrophoresis Power Supply EPS 500/400 is an accurately controlled power source of modern design that has been designed for safe and easy operation. The EPS 500/400 is designed to be used with the Pharmacia electrophoresis apparatuses (eg. the Gel Electrophoresis Apparatusus GE 2/4 and GE 2/4 LS, and the Flat Bed Electrophoresis apparatuses FBE Immuno and FBE 3000) or with any other suitable electrophoresis or immunoelectrophoresis chambers.

Description:

The Pharmacia Electrophoresis Power Supply EPS 3500 is a high quality, high precision and safe power supply for electrophoresis applications that require a maximum of 3500 volts. EPS 3500 is primarily designed for the following techniques: ● DNA sequencing ● IEF (Isoelectric focusing) EPS 3500 is also suitable for: ● ● ● ● ● SDS-PAGE (Polyacrylamide Gel Electrophoresis) Native PAGE Agarose electrophoresis Electroblotting DNA pulsed field electrophoresis Electrophoresis separations can be controlled by voltage, current or power. The EPS 3500 automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system. Two electrophoresis units can be connected to the EPS 3500 and run with the same programmed method at one time. Three programs can be saved.

Specifications:

• Output modes: Constant voltage, current or power
• Output voltage: 1 - 600 V in 1 V steps
• Output current: 1 - 400 mA in 1 mA steps
• Output power: 1 - 100 W in 1 W steps
• Program storage: 3 programmed methods
• Time: 1 min – 500 hr
• Volthours: 1 Vhr - 500kVhr
• Power outlets: 2 pairs, recessed 4 mm high voltage
• Operating temperature: 4°-40°C
• Power Consumption: 140 W
• Mains power: 100-120/220-240 VAC, 50-60 Hz
• Dimensions (W x D x H ): 25 x 39 x 10 cm
• Weight: 4.8 kg
• Safety features: Overload/short circuit detection
• Automatic cross-over
• Automatic recovery after power failure

Features:

• Frac-900 designed to work with AKTA series FPLC
• 95 places rack for 10-18mm tubes
• Fraction size 0.05 - 100 ml (0.001 ml increments)
• Time mode 0.01-9.999min, volume mode 0.01-9.999 ml
• Max flow rate 100 ml/min
• Peak detection and location capability, programmable collection modes

Powerful multifunctional control unit for chromatography systems. Programmable memory allows control of chromatographic procedures, modifications of separation parameters, performance of multidimensional chromatography, as well as evaluation and print out of the chromatogram data.

Specifications:

• Temperature Range: 4 to 40 °C
• Memory capacity: 50 Methods
• Dimensions: 16 L x 12 W x 4.7 H (in)

FRAC-100 (with its drop counting accessory) can collect fractions in a variety of different ways, based either on time, volume or drop counting, controlled via simple programs specified by the operator. For example, you can choose to collect uniform fractions of a fixed time period.

Specifications:

• Fraction Size Timing:01-99.99 min (0.01 min increments)
• Fraction Size Timing: 100-999.9 min (0.1 min increments)
• Fraction Size Timing: 1000-9 999 min (1 min increments) within an accuracy of ± 0.02%
• Fraction Size Volume: 01-99.99 ml (0.01 ml increments)
• Fraction Size Volume: 100-999.9 ml (0.1 ml increments)
• Fraction Size Volume: 1000-9 999 ml (1 ml increments) within and accuracy of ± 0.02%
• Maximum Drop Rate: 15 drops/sec
• Weight: 5 kg
• Dimensions: 30 x 29 x 39 cm
• Environment: +4 to +40°C, 20-95% relative humidity
• Environment: 84-106 kPa (840-1060 mbar) atmospheric pressure
• Peak Memory: up to 11 peaks
• Tubing:7 – 2 mm outer diameter

Description:

Single Path Monitor UV-1 is a dual beam flow through UV absorptiometer for monitoring the UV absorption of a flowing liquid at 254nm, 280 nm, or 405 nm, comes with the UV-1 control unit, optical unit, one 280 nm wavelength filter and one 280 nm converter, and with a flow cell with optical path length of either 3 mm or 10 mm.

Description:

A single channel peristaltic pump for use in liquid chromatography in the flow range from 0.6 to 500ml per hour.  The P-1 will interface with the programmable fraction collectors, FRAC-300 and FRAC-100 to provide accurate collection volume information to regulate fraction size.

Specifications:

• Standard tubing 1, 2.1, or 3.1mm ID
• 1.0 mm, 2.1mm, 3.1mm tubing is also available.
• Flow range 0.6 - 500 ml/hr
• Remote control

Description:

Single Path Monitor UV-1 is a dual beam flow through UV absorptiometer for monitoring the UV absorption of a flowing liquid at 254nm, 280 nm, or 405 nm, comes with the UV-1 control unit, optical unit, one 280 nm wavelength filter and one 280 nm converter, and with a flow cell with optical path length of either 3 mm or 10 mm.

Features:

Meet specific application needs with Thermo ScientificRefrigerator and Freezer Alarms, optional alarms for lab refrigerators and freezers.

Specifications:

• Exterior dimensions: 20 1/4 x 21 5/8 x 31 1/2 ” (W x D x H)
• Capacity: 4 cubic feet
• Temperature range: -15 to -25°C (-13 to 5°F)
• Defrost: Manual

Features:

• Disposable Presterile Bioreactor Chamber
• Fit Cellbags: 2x2L, 2x10L, 1x20L with KIT20 or 2x22L, 1X50L with KIT50
• Completely closed system
• Operate inside an incubator or on the benchtop

Includes:

• Electric Rocker Base for 20/50EHT
• Digital temperature control

Applications:

Monoclonal antibodies

The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

Anchorage-dependent cells

Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

Virus production

The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

Insect cell / Baculovirus

The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

Features:

• Disposable Presterile Bioreactor Chamber
• Completely closed system
• Operate inside an incubator or on the benchtop

Includes:

• Electric Rocker Base for 20
• Digital temperature control

Applications:

Monoclonal antibodies

The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

Anchorage-dependent cells

Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

Virus production

The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

Insect cell / Baculovirus

The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

Specifications:

• Range: 0 to 100% saturation with autozero.
• Standard single-bay enclosure or in optional RACK20.
• Reusable DOPROBE and TCPROBE purchased separately

Features:

Oxygen monitor

Specifications:

• Range: 0 to 100% saturation with autozero.