Applied Biosystems StepOne Plus Real-Time PCR

Cambridge Scientific ID: 27473 Category: Tag:


Includes Computer loaded w/ Step One Software


The StepOnePlus Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus Real-Time PCR System include:

  • Advanced software and instrumentation for performing a wide array of genomic assays
  • Sensitive 4-color optical LED recording system
  • Intuitive and robust software perfect for both first-time and advanced users
  • Simple and flexible instrument set-up and usage



  • Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
  • Detection Method: Primer-Probe Detection, SYBR
  • Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
  • Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
  • Format: 0.1 ml tubes, 8-tube strips, 96-well plate
  • Green Features: Energy efficient, Fewer resources used, Sustainable disposal
  • High Throughput Compatibility: Multiplexing
  • Optics: 4 emission filters, Blue LED excitation source, photodiode
  • Passive Reference Dye: No ROX, ROX (Pre-mixed), ROX (Separate Tube)
  • Peak Block Ramp Rate: 4.6°C⁄sec
  • Reaction Speed: Fast, Standard
  • Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
  • Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
  • Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
  • Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
  • Temperature Range (Metric): 4-100°C
  • Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
  • Thermal Cycling System: Peltier-Based System
  • Weight: 24 kg (53 lbs.)

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