Description
The BD LSRFortessa System’s innovative excitation and collection optics design reduces excitation losses and optimizes collection efficiency for increased sensitivity.
Maximum signal, minimum crosstalk-an innovative and proven platform for multicolor analysis
- The octagon and trigon-shaped optical pathways of collection optics maximize signal detection and increase sensitivity and resolution allowing you to identity dim and rare cell populations
- Can be configured with up to 5 lasers-blue,red, violet, UV and yellow-green. This flexibility in laser wavelengths allows you to optimize assay design using the latest fluorescent dyes and substrates.
- Can accommodate the detection of up to 19 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirements.
Specifications:
Optics
Excitation optics
- Excitation optical platform: The BD LSRFortessa optical layout allows for up to four lasers.
- Laser power: 355 nm: 20 mW, 405 nm: 50 mW, 488 nm: 50 mW, 640 nm: 40 mW
- Optical efficiency: Power loss at flow cell: <20% of specified laser power
- Flow cell design:
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- Rectangular quartz cuvette: Internal cross-section, 430 x 180 µm
- External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light.
- Fixed optical assembly with spatially separated laser beams.
Emission optics
- Optical coupling: The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
- Forward scatter detection: photodiode detector with a 488/10 bandpass filter
- Side scatter detector: Photomultiplier tube (PMT) with a 488/10 bandpass filter
Performance
- Fluorescence sensitivity:
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- FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
- PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
- PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy5)
- APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
- FITC and PE measurements performed using SPHERO Rainbow Calibration Particles (RCP-30-5A)
- PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K)
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- Fluorescence resolution: Coefficient of variation PI: Area of <3%, full G0/G1peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
- Fluorescence linearity: Doublet/singlet ratio of 1.95-2.05 for CEN stained with PI and excited with the 488-nm blue laser
- Forward and side scatter sensitivity: Enables separation of fixed platelets from noise.
- Forward and side scatter resolution: Scatter performance is optimized for resolving lymphocytes, monocytes, and granulacytes.
- Side scatter resolution: Enables separation of 0.5-µm beads from noise.
- Forward scatter PMT option: A forward scatter PMT upgrade is available for small particle detection through the special order program.
- Data acquisition rate: 40,000 events/s with beads
Fluidics
- Sample flow rates:
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- Front key panel provides three modes: RUN, STANDBY, and PRIME
- Continuously adjustable flow rate, plus three preset flow rates:
- LO: 12 µL/min
- MED: 35 µL/min
- HI: 60 µL/min
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- Standard fluidic reservoirs: One 8-L sheath container and one 10-L waste container provided.
- Recommended fluidic option: BD FACSFlow supply system: automated fluidics system, which includes a rolling art and two 20-L Cubitainer packages
Workstation:
- Operating system: Microsoft Windows XP Professional SP3
- Processor: Intel Core 2 Duo processor, 3.0 GHz
- RAM: HP 2 GB (2 x 1 GB) DDR2-800 ECCHard drivers:
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- HP 80 GB SATA/3 Gb/s 7,200 rpm HD (1st slot)
- HP 250 GB SATA/3 Gbs NCQ
- 7,200 rpm HD (2nd slot)
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- DVD drive: HP 16x DVD+/-RW, SuperMulti SATA
- Networking: Integrated Broadcom Gigabit 10/100/1000 ethernet, Broadcom 5751 NetXtreme Gigabit PCIE NIC Ethernet