BD Biosciences LSRFortessa Cell Analyzer

Cambridge Scientific ID: 29491 Category: Tag: Brands:

Description

The  BD LSRFortessa System’s innovative excitation and collection optics design reduces excitation losses and optimizes collection efficiency for increased sensitivity.  

Maximum signal, minimum crosstalk-an innovative and proven platform for multicolor analysis

  • The octagon and trigon-shaped optical pathways of collection optics maximize signal detection and increase sensitivity and resolution allowing you to identity dim and rare cell populations
  • Can be configured with up to 5 lasers-blue,red, violet, UV and yellow-green. This flexibility in laser wavelengths allows you to optimize assay design using the latest fluorescent dyes and substrates. 
  • Can accommodate the detection of up to 19 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirements. 

Specifications: 

Optics 

Excitation optics 

  • Excitation optical platform: The BD LSRFortessa optical layout allows for up to four lasers. 
  • Laser power: 355 nm: 20 mW, 405 nm: 50 mW,  488 nm: 50 mW, 640 nm: 40 mW  
  • Optical efficiency: Power loss at flow cell: <20% of specified laser power
  • Flow cell design: 
    • Rectangular quartz cuvette: Internal cross-section, 430 x 180 µm
    • External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light. 
    • Fixed optical assembly with spatially separated laser beams. 

Emission optics

  • Optical coupling: The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency. 
  • Forward scatter detection: photodiode detector with a 488/10 bandpass filter
  • Side scatter detector: Photomultiplier tube (PMT) with a 488/10 bandpass filter

Performance

  • Fluorescence sensitivity: 
      • FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
      • PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
      • PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE-Cy5)
      • APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
      • FITC and PE measurements performed using SPHERO Rainbow Calibration Particles (RCP-30-5A)
      • PE-Cy5 and APC measurements performed using SPHERO Ultra Rainbow Calibration Particles (URCP-38-2K)
  • Fluorescence resolution: Coefficient of variation PI: Area of <3%, full G0/G1peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Fluorescence linearity: Doublet/singlet ratio of 1.95-2.05 for CEN stained with PI and excited with the 488-nm blue laser
  • Forward and side scatter sensitivity: Enables separation of fixed platelets from noise.
  • Forward and side scatter resolution: Scatter performance is optimized for resolving lymphocytes, monocytes, and granulacytes. 
  • Side scatter resolution: Enables separation of 0.5-µm beads from noise. 
  • Forward scatter PMT option: A forward scatter PMT upgrade is available for small particle detection through the special order program. 
  • Data acquisition rate: 40,000 events/s with beads

Fluidics

  • Sample flow rates: 
      • Front key panel provides three modes: RUN, STANDBY, and PRIME
      • Continuously adjustable flow rate, plus three preset flow rates: 
      • LO: 12 µL/min
      • MED: 35 µL/min
      • HI: 60 µL/min
  • Standard fluidic reservoirs: One 8-L sheath container and one 10-L waste container provided. 
  • Recommended fluidic option: BD FACSFlow supply system: automated fluidics system, which includes a rolling art and two 20-L Cubitainer packages

Workstation: 

  • Operating system: Microsoft Windows XP Professional SP3
  • Processor: Intel Core 2 Duo processor, 3.0 GHz
  • RAM: HP 2 GB (2 x 1 GB) DDR2-800 ECCHard drivers: 
      • HP 80 GB SATA/3 Gb/s 7,200 rpm HD (1st slot)
      • HP 250 GB SATA/3 Gbs NCQ
      • 7,200 rpm HD (2nd slot)
  • DVD drive: HP 16x DVD+/-RW, SuperMulti SATA
  • Networking: Integrated Broadcom Gigabit 10/100/1000 ethernet, Broadcom 5751 NetXtreme Gigabit PCIE NIC Ethernet

Contact Us

Thank you for your interest in the BD Biosciences LSRFortessa Cell Analyzer (SKU:29491) To speak with a representative, please fill out the contact form below or call us directly.

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