Description
Droplet Digital PCR (ddPCR) is a digital polymerase chain reaction method based on water-oil emulsion droplet technology. ddPCR uses a combination of microfluidics and proprietary surfactant chemistries to divide each sample into water-in-oil droplets. The technology uses reagents and workflows similar to those used for most standard TaqMan probe-based assays, and provides absolute quantification of nucleic acid target sequences by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions.
Features and Benefits:
Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:
- Guides setup and loading with large, color touch-screen interface and LED lighting
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
- Provides operational flexibility:
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- User-definable plate layout with increments of 8 wells
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
- Compatible with both QX600 and QX200 ddPCR workflows
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Specifications:
- Weight: 100 lb (45.4 kg)
- Size (W x D x H): 26 x 22 x 26 in (66 x 56 x 66 cm)
- Automated Droplet Generator: Instrument used to generate standard droplets
- Cooling block accessory: Prevents evaporation during droplet generation
- Power cord: U.S. standard power cord
- Oil purge reservoir: Collects oil waste from priming and flushing
- ddPCR 96-Well Plates: Clear well/clear shell semi-skirted plates
- DG32 Droplet Generation Cartridges: Microfluidic cartridge with gasket for the AutoDG
- Automated Droplet Generation Oils:
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- Automated droplet generation oils for the AutoDG
- Automated Droplet Generation Oil for Probes
- Automated Droplet Generation Oil for EvaGreen
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- Droplet Reader Oil: ddPCR droplet reader oil for QX600 and QX200 instruments and systems
- Pipet Tips: Pipet tips for the AutoDG
- Pipet Tip Bins: Pipet tip waste bins for the AutoDG
