Description
ForteBio’s Octet RED96e system is a multi-functional, label-free, real-time analysis instrument. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity. The Octet RED96e system is a 96-well characterization of protein-protein and protein-small molecule binding kinetics, and for the determination of protein concentrations and titer. Popular industrial applications include expression screening, on- and off-rate screening, epitope binning, cell line development and bioprocess optimization.
Specifications:
- Detection technology: Bio-Layer Interferometry (BLI)
- Biosensor type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the sensor tray
- Information provided:
- Yes/No binding
- Kinetic and affinity analysis (kobs, ka, kd, KD)
- Specific and selective detection of molecules, even in crude samples
- Relative and absolute quantitation of specific proteins in crude matrices or purified samples
- Data presentation:
- Plots displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
- Concentration data analysis including calibration curves and output of tabulated concentration data
- Tabulated kinetic data
- Epitope binning and cross-blocking matrices and trace overlays
- Sample types: Proteins, antibodies, peptides, DNA, RNA,
liposomes, bacterial cells, viruses, mammalian cells, small molecules in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates - Number of spectrometers: 8
- Maximum simultaneous reads: 8
- Data collection rate: 2, 5, or 10 Hz
- Sample position and format: 1 standard 96-well, black, flat bottom microplate
- Sample volume: 180–220 μL/well, non-destructive testing
- Orbital flow capacity: Static or 100–1500 rpm
- Analysis temperature range: 15–40°C, 1°C increments
- Workflow: Up to 8 assays in parallel; up to 96 assays
per 96-well plate - Molecular weight detection: >150 Da
- Analysis time per sample: Real-time kinetic binding experiments from 5 minutes to 4 hours, or up to 12 hours with
the evaporation cover - Association rate constant (k2): 101-107 M-1s-1
- Dissociation rate constant (k2): 10-6-0.1 s-1
- Affinity (KD) constant: 1 mM-10 pM
- Baseline noise: ≤ 3.5 pm (RMS)
- Baseline drift: ≤ 0.1 nm/hour
- Workflow: Up to 8 assays in parallel; up to 96 assays per 96-well plate
- Analysis time per sample: Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
- Direct quantitation range for human IgG with Protein A Biosensor: 0.05-2000 ug/mL
- Dimensions (H x W x D): 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
- Weight: 72 lb (32.7 Kg)
- Electrical requirements: Mains: 100-120/200-240 VAC, 50/60 Hz, 4 A max
- Power consumption: 200 W (300 W peak)
- PC operating systems: Windows® 10 Professional, 64-bit, Windows 7 Professional, 64-bit, Windows 7 Professional, 32-bit
- Safety standards: CE, Nemko
