Used Lab Equipment

High-performance, fully automated imaging has made the AlphaImager HP our most popular platform. The system's application versatility not only includes a wide range of fluorescent and colorimetric applications, but can also be expanded to chemiluminescence as laboratory needs change.

Specifications:

• Camera Resolution: 4 megapixels
• A/D: 12-bit
• Lens: 8 – 48 mm, f/1.2 Zoom, motorized
• Maximum Field of View: 42 x 31 cm
• Filter Positions: 5, motorized
• Size: 15 x 20 x 24 in
• Weight: 80 lbs

Description:

High-content screening (HCS) or high-content analysis (HCA) requires an instrument designed to extract the maximum information from your sample in a robust and reproducible manner with the speed for high-throughput analysis. The entire Thermo Scientific portfolio of HCA instruments helps deliver on those parameters and includes:

• Superior illumination—the LED light engine reduces intensity fluctuations and optical component wear, eliminates moving parts, and reduces both integration and channel switching times
• Rapid data analysis—Thermo Scientific HCS Studio software analyzes your images in real time, so that you collect all the data you need to make decisions that count
• Reagent compatibility—Thermo Scientific HCS platforms build on the expertise of Molecular Probes in reagent development with exquisite fluorescent tools for interrogating the biology of the cell

Specifications:

• Camera: Photometrics X1 Camera with 4.54um/pixel
• Live cell: Optional HCA On-Stage Incubator with Kinetic scheduling of acquisition
• Software: HCA Studio 3.1 Cell Analysis Software. Optional client software available
• Database: STORE Express Image and Database Management Software Included, Scalable SQL/Oracle database options available

Includes Computer loaded w/ Image Lab Software

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.

Features and Benefits

• Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection
• Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting
• High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation
• Superior Image Quality — exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds
• Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height; automated hands-free operation ensures consistent, reproducible, and high-throughput performance

Applications     

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation:Yes

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26 x 35 cm
• Maximum image area: (For standard UV-excited gels) 25 x 26 cm
• Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and epi-blue LED modules available
• Illumination control: 8 modes available. Trans-UV, epi-white, and no illumination for chemiluminescence are standard; epi-red, epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are optional
• Detector: Supercooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Cooling system: Peltier
• Camera cooling temperature: -30°C absolute and regulated
• Filter holder: 6 positions (5 for filters, 1 without filter for chemiluminescence)
• Emission filters: 1 included (standard), 4 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific; for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

Operating Ranges       

• Operating voltage: 110/115/230 VAC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity, noncondensing: <70%

Automation Capabilities         

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
• Image flat fielding**: Dynamic; precalibrated and optimizor every application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Features: 

Features for the Photometrics Cool SNAP K4: 

• 20-MHz readout: High-speed, high-sensitivity image capture
• 2048 x 2048 imaging array: Resolves fine detail
• 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
• Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
• Flexible binning and readout: Increases light sensitivity while increasing the frame rate
• 12-bit digitization: Quantifies bright and dim signals in the same image
• Thermoelectric cooling: Long integration times for higher sensitivity
• Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
• C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
• Acquisition software: Captures, analyzes, and saves high-resolution images
• PCI interface: High-bandwidth, uninterrupted data transfer
• PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
• CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
• CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
• Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
• Read noise: ≤10 e- rms @ 20 MHz
• Nonlinearity: <1%
• Digitizer type: 12 bits @ 20 MHz
• CCD temperature: -25ͦC (regulated)
• Dark current : 0.1 e-/p/s @ -25ͦC
• Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
• I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation: Yes  

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26X 35 cm
• Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
• Illumination control: 3 modes (trans-UV, trans white, epi-white)
• Detector: super cooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Filter holder: 3 positions (2 for filters, 1 without filter)
• Emission filters: 1 included (standard), 3 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific, for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

 Operating Ranges         

• Operating voltage: 110/115/230 V AC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity: <70% noncondensing

 Automation Capabilities            

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus: Precalibrated focus for any zoom setting or sample height
• Image flat fielding: Dynamic; precalibrated and optimized per application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Features:

Chemi Genius 2 Bio Imaging System

Specifications:

• Hamamatsu C4742-80-12AG ORCA-ER Camera Controller

• Hermetic Vacuum Sealed Head

• High Resolution of 1.37 Million Pixels

• Exposure Time up to 10 Seconds

• Progressive Scan Interline CCD Chip with No Mechanical Shutter

• Binning Function

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

The NEW super compact and intuitive live cell imaging system, Celloger Nano – got everything you need to perform your sophisticate laboratory works. Equipped with exceptional fluorescence and auto-focusing technology, precise stage controller, and user-friendly software, accelerates your cell-based research works. Expand your cell-based research through our multifunction live cell imaging system, Celloger Nano.

Description:

• One color fluorescence (GFP or RFP) and brightfield imaging technology
• Compact size that easily fits into standard CO2 incubators
• High quality system yet cost-effective
• Compatible with various cell and tissue culture vessel types
• Intuitive UI/UX and easy to acquire confluency data
• Increased focus speed and reproducibility with reliable autofocusing function
• Movie maker provides one-click easy way to make a time-lapse video

 Features:

• Applications: fluorescence and colorimetric
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: high sensitivity, high resolution scientific grade CCD camera with 2.0 megapixel resolution, extendable to 6.0 megapixels
• Lens:5-75 f/1.2
• Overhead Light Selector: White light, 365 nm UV, 254 nm UV
• Flters: ethidium bromide, SYBR Green, SYBR Gold
• Visible color stains: Coomassie, Silver
• Allows to visualize chemiluminscent reactions
• Manual lift platform, 3 emission filters, white light plate, overhead UV, white light and blue light
• Transilluminator: Choice of UV transilluminator models

Includes Computer Loaded with Software

The FluorChem HD2 System provides a complete array of image acquisition modes for common fluorescent and chemiluminescent applications.  The FluorChem HD2 System delivers higher sensitivity and a broader dynamic range than film can provide at similar exposure times. Captured images are saved digitally, making them easy to import into presentations and reports

Features:

• Applications Types: Chemiluminescent, Colorimetric, uv, Fluorescent
• Camera Resolution: 19 megapixels (2048 x 2048)
• a/D: 16-bit
• Recommended lens: 50 mm, f/0.95 Fixed, manual or motorized
• Pixels: 4 x 7.4 μm
• Filter Positions: 5, motorized
• Size: 20" W x 15" D x 39.5" h (51 x 38 x 100 cm)
• Weight: 100 lbs (45.4 kg)

Features:

The ImageQuant LAS 4000 is a camera system for producing digital images of gel or membrane samples or films. It is used together with the ImageQuant LAS 4000 Control Software to:

• Expose chemiluminescent samples or membranes.

• Expose fluorescent samples using epi (incident) or trans (transmitted) light sources, for example gels stained by ethidium bromide (EtBr). The ImageQuant LAS 4000 can be supplied with NIR, Red, Green, Blue and UV epi lights.

• Expose dye-stained gels and membranes or films using epi or trans illumination.

• Expose a sample repeatedly, incrementally or by programming exposure schemes.

Specifications:

• CCD: 3 200 000 pixels (Fujifilm super CCD
• Cooling temperature: -25°C
• Exposure time: 1/100 seconds to 2 hours (Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software.)
• Lens: High-sensitivity lens F0.85 43 mm, Wide view lens F1.8 24 mm
• Number of gradations:

Recorded image: 16 bit

Focusing: 8 bit