Used Lab Equipment

High-performance, fully automated imaging has made the AlphaImager HP our most popular platform. The system's application versatility not only includes a wide range of fluorescent and colorimetric applications, but can also be expanded to chemiluminescence as laboratory needs change.

Specifications:

• Camera Resolution: 4 megapixels
• A/D: 12-bit
• Lens: 8 – 48 mm, f/1.2 Zoom, motorized
• Maximum Field of View: 42 x 31 cm
• Filter Positions: 5, motorized
• Size: 15 x 20 x 24 in
• Weight: 80 lbs

Features: 

• 20-MHz readout: High-speed, high-sensitivity image capture
• 2048 x 2048 imaging array: Resolves fine detail
• 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
• Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
• Flexible binning and readout: Increases light sensitivity while increasing the frame rate
• 12-bit digitization: Quantifies bright and dim signals in the same image
• Thermoelectric cooling: Long integration times for higher sensitivity
• Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
• C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
• Acquisition software: Captures, analyzes, and saves high-resolution images
• PCI interface: High-bandwidth, uninterrupted data transfer
• PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
• CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
• CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
• Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
• Read noise: ≤10 e- rms @ 20 MHz
• Nonlinearity: <1%
• Digitizer type: 12 bits @ 20 MHz
• CCD temperature: -25ͦC (regulated)
• Dark current : 0.1 e-/p/s @ -25ͦC
• Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
• I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation: Yes  

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26X 35 cm
• Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
• Illumination control: 3 modes (trans-UV, trans white, epi-white)
• Detector: super cooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Filter holder: 3 positions (2 for filters, 1 without filter)
• Emission filters: 1 included (standard), 3 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific, for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

 Operating Ranges         

• Operating voltage: 110/115/230 V AC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity: <70% noncondensing

 Automation Capabilities            

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus: Precalibrated focus for any zoom setting or sample height
• Image flat fielding: Dynamic; precalibrated and optimized per application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

Features:

• Transillumination White: For protein gels, autorads, film, plates, flasks
• Transillumination UV: For fluorescent gels such EtBr, SYPRO Red, SYBR Safe, etc.
• Reflective White: For colorimetric blots and membranes
• For chemiluminescence
• High-performance, high resolution, CCD camera
• Manual f/2.8 28-70mm zoom lens
• 92 Megapixel Resolution
• Advanced Camera with 16-bit 1MHz Digitization
• Regulated Cooling and Low Noise Readout
• Multiple Image Acquisition Modes for a Wide Range of Applications

Includes Computer Loaded with Software

The FluorChem HD2 System provides a complete array of image acquisition modes for common fluorescent and chemiluminescent applications.  The FluorChem HD2 System delivers higher sensitivity and a broader dynamic range than film can provide at similar exposure times. Captured images are saved digitally, making them easy to import into presentations and reports

Features:

• Applications Types: Chemiluminescent, Colorimetric, uv, Fluorescent
• Camera Resolution: 19 megapixels (2048 x 2048)
• a/D: 16-bit
• Recommended lens: 50 mm, f/0.95 Fixed, manual or motorized
• Pixels: 4 x 7.4 μm
• Filter Positions: 5, motorized
• Size: 20" W x 15" D x 39.5" h (51 x 38 x 100 cm)
• Weight: 100 lbs (45.4 kg)

The 810-UNV Image Eraser is used to erase latent images on phosphor storage screens to allow screen re-use.

Specifications:

• Screen Dimensions: 16" x 18"

Includes Computer loaded w/ Image Lab Software

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.

Features and Benefits

• Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection
• Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting
• High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation
• Superior Image Quality — exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds
• Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height; automated hands-free operation ensures consistent, reproducible, and high-throughput performance

Applications     

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation:Yes

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26 x 35 cm
• Maximum image area: (For standard UV-excited gels) 25 x 26 cm
• Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and epi-blue LED modules available
• Illumination control: 8 modes available. Trans-UV, epi-white, and no illumination for chemiluminescence are standard; epi-red, epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are optional
• Detector: Supercooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Cooling system: Peltier
• Camera cooling temperature: -30°C absolute and regulated
• Filter holder: 6 positions (5 for filters, 1 without filter for chemiluminescence)
• Emission filters: 1 included (standard), 4 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific; for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

Operating Ranges       

• Operating voltage: 110/115/230 VAC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity, noncondensing: <70%

Automation Capabilities         

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
• Image flat fielding**: Dynamic; precalibrated and optimizor every application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Includes:

• Cabinet & Camera
• Computer With Software
• Manual

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

Specifications:

• CCD Resolution (HxV): 1,392 x 1,040µm
• Pixel density: 65,536 gray levels
• Imaging area: 25 x 26 cm
• Camera cooling temperature: -50°C
• Camera cooling system: Peltier
• Dynamic range: 4 orders of magnitude
• Motorized zoom lens: C-mount, f/1.2, 12-75 mm
• Illumination modes: Epi-white, trans-UV (302 nm), and white

Specifications:

• Dimensions (W x D x H): 20.25” x 15.5” x 17.5” (51.4 x 39.4 x 44.5 cm)
• Weight: 36.3 kg
• Working Environment: power up temperature between 18ºC to 30ºC.
• Humidity: 10% to 85% relative humidity (non-condensing)
• Power Supply: 24-volt external power supply compatible with 100–240 V~; +/-10% at 50–60 Hz
• Power Consumption: 130 Watts maximum
• Temperature control: 4ºC over ambient to 45ºC
• Temperature variation: ±0.5ºC across the plate at 37ºC

View film negatives and positives, autoradiographs, X-ray film, microtiter plates, and stained gels. Glass surface is permanently double safety-sealed into the base for durability. Scratch- and chemical-resistant work surface can be easily cleaned. Housing is finished with an epoxy-powder paint for easy cleaning and maintenance.

Specifications:

• Weight: 12 lbs.

• Measurements: 22in x 12in x 4in

• Electrical: 115V, 60Hz, 3 Amps

FUJIFILM BAS IPE IP IMAGING PLATE ERASER

Features:

• AC 100-120/200-240V
• 0/1.0 A
• 50/60Hz