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Monoclonal antibodies
The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.
Anchorage-dependent cells
Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.
Virus production
The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.
Insect cell / Baculovirus
The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.
Description:
High-content screening (HCS) or high-content analysis (HCA) requires an instrument designed to extract the maximum information from your sample in a robust and reproducible manner with the speed for high-throughput analysis. The entire Thermo Scientific portfolio of HCA instruments helps deliver on those parameters and includes:
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Hertherm IGS180 Incubator
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The Incu-Mixer MP heated microplate mixer is designed to provide an optimal method for vortexing and incubating microplates. Simultaneous heating from both the bottom surface and the lid provide temperature uniformity throughout the sample. Additionally, the heated lid minimizes evaporation and prevents condensation. A compact, 2mm orbit ensures thorough mixing in microplates without splashing. Speed, time and temperature are all displayed in real time on the large LCD screen while an internal validation system constantly monitors all parameters for accuracy. Designed for application flexibility, the Incu-Mixer series is available in two, and four-plate capacities, both capable of accepting all standard plates up to 40mm high. Independent time, temperature and speed controls allow the mixer to function as 3 machines in one; a multi-plate incubator, vortexer, or a combination of the two. The Incu-Mixer MP series is ideal for a wide variety of molecular biology applications including immunochemical reactions, enzyme and protein analysis, and microarray analysis.
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The Incu-Mixer MP heated microplate mixer is is designed to provide an optimal method for vortexing and incubating microplates. Simultaneous heating from both the bottom surface and the lid provide temperature uniformity throughout the sample. Additionally, the heated lid minimizes evaporation and prevents condensation. A compact, 2mm orbit ensures thorough mixing in microplates without splashing. Speed, time and temperature are all displayed in real time on the large LCD screen while an internal validation system constantly monitors all parameters for accuracy. Designed for application flexibility, the Incu-Mixer series is available in two, and four-plate capacities, both capable of accepting all standard plates up to 40mm high. Independent time, temperature and speed controls allow the mixer to function as 3 machines in one; a multi-plate incubator, vortexer, or a combination of the two. The Incu-Mixer MP series is ideal for a wide variety of molecular biology applications including immunochemical reactions, enzyme and protein analysis, and microarray analysis.
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The Model 400 Lumascope iVue is a true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent Lumascope. It takes the advantage of a color CMOS camera. The iVue provides powerful, low-cost, time-lapse microscopy in your incubator.
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With a square footprint of under 5 inches, the MiniMag is the most compact stirrer in the Benchmark line. Despite its modest dimensions, the 3.4 inch square work surface accepts a wide variety of beakers, flasks, bottles and other laboratory vessels for stirring volumes as large as 1.5 liters. The stirring speed is continuously adjustable from 100 to 1,500rpm. For temperature sensitive samples, the MiniMag is incubator and cold-room safe for use from +4°C to +45°C.
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Compact, universal small shaker suitable for shaking tasks with all small vessels and microtiter plates.
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Compact, universal small shaker suitable for shaking tasks with all small vessels and microtiter plates.
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Compact, universal small shaker suitable for shaking tasks with all small vessels and microtiter plates.
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Multiskan Spectrum makes getting results easier, replacing separate spectrophotometers and microplate reading instruments by providing both functions in one convenient, compact instrument. The Multiskan Spectrum is truly two instruments in one, combining both cuvette reading capabilities and advanced microplate reading in one convenient, compact instrument. You can easily take quick absorbance measurements in a cuvette between the cycles of a microplate enzyme kinetics experiment.
For maximum flexibility, Multiskan Spectrum accepts all microplate for mats from 6- to 384-wells, as well as cuvettes from standard to ultra micro capacities in glass, plastic, or quartz. You can take multiple photometric readings per well on large format plates, or single measure ments requiring just 30 μl in 384-well plates.
Scanning range of 200 – 1000 nm. The built-in monochromator makes it simple to select any wavelength from 200 nm to 1000 nm in 1 nm steps without ever changing a filter. Sample absorbances can be scanned over the whole spectrum either in microplates or cuvettes.
Typical applications of the Multiskan Spectrum
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The SCI-RD-E provides a gentle but effective mixing of biological samples in 1.5ml to 50ml micro tubes, used in a variety of applications including immune precipitations, prevention of blood coagulation, latex diagnostics etc.
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