Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Flow Cytometers and Cell Sorters. Some of our popular manufacturers include Beckman-Coulter, BD Biosciences (Becton-Dickinson) and Guava.

Amersham Biosciences Ge: 1-15 of 16

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Amersham Biosciences GE A-900 FPLC Autosampler

Amersham Biosciences GE A-900 FPLC Autosampler

Cambridge ID #: 6705

Features:

  • Loop volume 5-1000µl
  • 96 vials 1.5 ml vial capacity
  • A-900 autosampler designed for use with AKTA
  • Syringes 100 - 1000 µl
  • Built-in compressor
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Amersham Biosciences GE AKTA FPLC FPLC System

Amersham Biosciences GE AKTA FPLC FPLC System

Cambridge Scientific ID #'s: 21453, 16947, 16751, 21302

AKTA FPLC system is a fully automated liquid chromatography system designed for research scale purification of proteins, wherein proteins of various sizes can be readily purified using different kinds of columns.

 

Features:

  • P-920 Pump
  • UPC-900
  • Frac-900 Fraction Collector
  • INV-907 Injection Valve
  • M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
  • Box 900: Storage compartment and Solvent Tray
  • Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 
  • Manuals
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Amersham Biosciences GE AKTA Prime FPLC System

Amersham Biosciences GE AKTA Prime FPLC System

Cambridge Scientific ID #'s: 20233, 20127, 20411

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

 

 

Features:

  • Fraction collector
  • pH Electrode
  • UV Monitor
  • Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 
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Amersham Biosciences GE AKTA Prime Plus FPLC System

Amersham Biosciences GE AKTA Prime Plus FPLC System

Cambridge ID #: 21454

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

 

 

Features:

  • Fraction collector
  • pH Electrode
  • UV Monitor
  • Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 
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Amersham Biosciences GE AKTA Purifier FPLC System

Amersham Biosciences GE AKTA Purifier FPLC System

Cambridge Scientific ID #'s: 21301, 14009, 14179, 14258, 20416

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

 

Features:

  • Box 900: Storage compartment and Solvent Tray
  • CU-950: Communications Module
  • P-950 sample pump
  • P-900: System Pump Model with Four Pump Heads in Two Pump Modules
  • M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
  • UV-900: Variable, Multiwavelength UV-Vis detector
  • pH/C-900: pH and Conductivity Detector
  • Frac-920: Fraction Collector
  • Model INV-907: Injection Valve
  • Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
  • Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 
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Amersham Biosciences GE AKTA Purifier 10 FPLC System

Amersham Biosciences GE AKTA Purifier 10 FPLC System

Cambridge ID #: 16287

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

 

Features:

  • Box 900: Storage compartment and Solvent Tray
  • CU-950: Communications Module
  • P-950 sample pump
  • P-900: System Pump Model with Four Pump Heads in Two Pump Modules
  • M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
  • UV-900: Variable, Multiwavelength UV-Vis detector
  • pH/C-900: pH and Conductivity Detector
  • Frac-920: Fraction Collector
  • Model INV-907: Injection Valve
  • Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
  • Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 
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Amersham Biosciences GE AKTAxpress Single Module FPLC System

Amersham Biosciences GE AKTAxpress Single Module FPLC System

Cambridge ID #: 11554

 

ÄKTAxpress Module

 

ÄKTA xpress is a chromatography system designed for automated, multistep protein purification of both single and multiple samples.

  • Unattended purification: Built-in purification knowledge combines software, hardware, and prepacked columns to eliminate time-consuming manual tasks.
  • Highest possible purity: Optimized protocols with up to four steps will deliver tagged proteins of near 100% purity. Built-in cleaning procedures between MAb samples to minimize carry over
  • High productivity: Each module can purify up to four proteins. The modular design offers adaptability to meet increased throughput needs.
  • Yield : The system is capable of purifying target proteins in yields varying from 1 to 50+ mg.
  • Akta Express has a Small footprint: A compact design allows the system to occupy minimal space on the bench or in the cold room.

 

ÄKTAxpress Module

  • Complete Packsize: 1 Piece
  • Application: Unattended multistep purification of tagged proteins and antibodies
  • Flow Rate: 0.1-65 ml/min
  • Operating Pressure Max.: 3 MPa (435 psi)
  • Tubing i.d. [Flow Path]: 1 mm
  • Single Wavelength Detection: Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm.
  • Multiple Wavelength Detection: No
  • Flow Cell Path Length: 2 mm
  • Multiple Sample Injection: Yes, up to 4 samples
  • Autosampler Injection: No
  • Automatic Buffer Preparation: No
  • Buffer Selection: 10 buffer inlets
  • Multi-Step Purification: Yes, up to 4 steps
  • Relative Humidity: 20%-95% (non condensing)
  • pH Stability Long Term: 2-12
  • pH Stability Short Term: 1-14
  • Viscosity: max 5 cP
  • Width: 250 mm
  • Height: 660 nm
  • Depth: 600 mm
  • Weight: 30 kg
  • Voltage: 100-120/220-240 VAC
  • Power Consumption: 120 VA
  • Protection Class: IP 20
  • Pump Type: Piston pump, metering type
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Amersham Biosciences GE Ettan IPGphor II Electroporation SystemImage Coming Soon

Amersham Biosciences GE Ettan IPGphor II Electroporation System

Cambridge ID #: 8998

Description:

The Ettan IPGphor II from GE Healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2D gels. The manufacturer claims that the system provides ‘reproducibility, reliability, speed, accuracy, high protein loading capacity, and high throughput’, and in my experience, the system really stands up to their claim. To begin with, the system is very compact and can be housed on a tabletop in any corner of the lab. It is perfectly silent and occupies little space. The system requires an ambient, dust-free operating environment; however, we keep the instrument in a well air-conditioned room. The system contains gold-plated copper electrodes that provide the necessary contact to the 1D strips for carrying out isoelectric focusing. A very high voltage is applied between the electrodes and, therefore, caution should be used while handling the instrument. As a safety feature, the electrodes are automatically switched off when anyone opens the lid of the instrument.

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Amersham Biosciences GE FRAC-900 FPLC Fraction Collector

Amersham Biosciences GE FRAC-900 FPLC Fraction Collector

Cambridge Scientific ID #'s: 7632, 21455, 6707, 5798

Features:

  • Frac-900 designed to work with AKTA series FPLC
  • 95 places rack for 10-18mm tubes
  • Fraction size 0.05 - 100 ml (0.001 ml increments)
  • Time mode 0.01-9.999min, volume mode 0.01-9.999 ml
  • Max flow rate 100 ml/min
  • Peak detection and location capability, programmable collection modes
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Amersham Biosciences GE Frac-950 FPLC Fraction Collector

Amersham Biosciences GE Frac-950 FPLC Fraction Collector

Cambridge Scientific ID #'s: 19075, 13340, 21300, 16288, 21299, 16150, 13653, 21500, 14492

Includes:

  • Manual
  • Vial Holder rack
  • Power Cord

 

Specifications:

  • Maximum flow rate: 100 ml/min
  • pH stability range: 1 to 13, 1 to 14 (<1 day exposure)

Fraction size

  • Volume mode: to 99999.99 ml
  • Time mode: to 99999.99 min

No spillage range for DropSync

  • Microplates: 0 to 1.0 ml/min
  • 12 mm rack: 0 to 1.5 ml/min
  • 18 and 30 mm rack: 0 to 2.0 ml/min

No spillage range for Accumulator

  • All racks: 0 to 100 ml/min
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Amersham Biosciences GE REC 112 RecorderImage Coming Soon

Amersham Biosciences GE REC 112 Recorder

Cambridge ID #: 11863

Description:

The Amersham Pharmacia Biotech Biotech REC 112 Dual Channel Data Chart Recorder is a versatile and accurate way to track laboratory information.

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Amersham Pharmacia Biotech Frac-950 FPLC Fraction Collector

Amersham Pharmacia Biotech Frac-950 FPLC Fraction Collector

Cambridge ID #: 21833

Includes:

  • Manual
  • Vial Holder rack
  • Power Cord

 

Specifications:

  • Maximum flow rate: 100 ml/min
  • pH stability range: 1 to 13, 1 to 14 (<1 day exposure)

Fraction size

  • Volume mode: to 99999.99 ml
  • Time mode: to 99999.99 min

No spillage range for DropSync

  • Microplates: 0 to 1.0 ml/min
  • 12 mm rack: 0 to 1.5 ml/min
  • 18 and 30 mm rack: 0 to 2.0 ml/min

No spillage range for Accumulator

  • All racks: 0 to 100 ml/min
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BD Biosciences Accuri C6 w/ csampler Flow Cytometer

BD Biosciences Accuri C6 w/ csampler Flow Cytometer

Cambridge ID #: 21140

Features:

  • BD CSampler- 96 well plate and 24 tube rack automated sampler for BD Accuri C6 flow cytometer  
  • Blue and red laser
  • Two light scatter detectors
  • Optical filters such as FITC, PE, PerCP and APC
  • A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use
  • Optional filters and the Selectable Laser Module expand the available fluorochrome combinations
  • Compact system and portable weight (13.6 Kg)
  • Intuitive interface of the software
  • Measures: 11 x 14.75 x 16.5 inches (H x W x D) (27.9 x 37.5 x 41.9 cm)
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BD Biosciences FacsAria IIu Cell Sorter

BD Biosciences FacsAria IIu Cell Sorter

Cambridge Scientific ID #'s: 14996, 17981

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

 

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

 

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

 

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

 

Includes:

  • 3 lasers
  • Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
  • Computer w/ FACSDiva 8 Software
  • ACDU
  • 2 monitors
  • Fluidics Cart
  • Temperature Control
  • Lauda Chiller

 

Performance

Fluorescence Sensitivity

  • Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
  • FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

  • Coefficient of variation (CV)
  • PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

  • Doublet/singlet ratio:
  • CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

  • Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

  • Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

  • Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.
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BD Biosciences FacsCanto II - 3 Laser Flow Cytometer

BD Biosciences FacsCanto II - 3 Laser Flow Cytometer

Cambridge ID #: 12160

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 2 laser, Blue(488) and Red (633)
  • Laser
  • 6 PMTs unit and side scatter
  • All Necessary Cables
  • Manuals

 

Optics Specifications:

  • Lasers
    • Air-cooled:
    • 488-nm solid state, 20-mW laser output
    • 633-nm HeNe, 17-mW laser output
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 0 μm
  • SSC Resolution: 5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
  • Blue Laser Dyes
    • FITC, PE, PerCP or PerCP-Cy™5.5,
    • PE-Cy7 (525, 575, 678 or 695, 785 nm)
  • Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
  • Detector Bands
    • Blue Laser: 530/30; 585/42; >670; 780/60 nm
    • Red Laser: 660/20; 780/60 nm
  • Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF
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