Used Lab Equipment

The easy-to-use Model 2110 Fraction Collector provides multiple collection modes for chromatographic separations.

Features:

• Time or drop collection modes (or volume collection mode when connected to the Model EP-1 Econo Pump, BioLogic LP System, or BioLogic DuoFlow System)
• Collection of 1 drop (~50 µl) to 9 ml fractions in 80 test tubes or microtubes (with optional adaptor)
• Small chromatography columns can be mounted to drop-forming arm to minimize dead volume
• Manual-advance tube changes
• Coldroom compatibility
• Small footprint of 24 x 33 cm

Specifications:

• Capacity: Eighty 13 x 100 mm glass, polypropylene, or polystyrene test tubes, or 1.5 ml microtubes when using optional micro tube adaptor
• Collection basis: 1–999 drops in 1 drop increments, 0.05–9.99 min in 0.01 min increments, 10.0–99.9 min in 0.1 min increments
• Construction: Case and carousel, polypropylene; front panel, hard-coated polycarbonate
• Drop former: Silicone rubber
• Waste outlet tube: Polyurethane
• Operating temperature: 4–40°C
• Safety: Meets IEC 61010 and CSA 22.2 certification
• Dimensions (W x D x H): 24 x 33 x 25 cm

Description:

The Bio-Rad Model 3000xi Computer Controlled Power Supply produces a constant voltage to 3,000 volts, constant current to 300 mA, and constant power to 400 watts. This fully switching, microprocessor controlled unit may be used with any electrophoresis instrument. The high outputs make the Model 3000xi Power Supply ideal for electrofocusing, DNA sequencing, and isotachophoresis. The supply is useful as a general purpose instrument, and is recommended for SDS-PAGE electrophoresis, two-dimensional electrophoresis, native gel electrophoresis, electrophoretic blotting, and DNA/RNA separations. The 3000xi Power Supply is a fully programmable and computerized instrument that incorporates several unique features. The supply offers four operating modes: standard, time, volt-hour, and step. The operator has a choice of running electrophoresis manually, for a set period of time, or for a set number of volt-hours. These parameters can be combined using the step mode. While operating in any one of the four modes, the user simply enters the desired power conditions and begins the run. The operation parameters are displayed on the LCD. Output voltage, current, and power are displayed on the LED display. Electrophoresis running conditions can also be programmed. Up to 10 routines can be saved in the Model 300xi Power Supply's memory, where they can be recalled at any time. This is especially useful when using the special step mode which allows three separate electrophoresis running conditions to be programmed simultaneously. A single gel can then be run safely at three different power conditions without the need for constant monitoring.

Features:

• Output Voltage: 25-3000 Volts DC
• Output Current: 0-300 mA
• Output Power: 0-400 W
• Overload and Short Protection: Circuit breaker
• Leakage Detection: Lamp and alarm >500 µA
• Operating Temperature: 0-40°C
• Dimensions (W x H x D): 40 x 32 x 11.5 cm
• Weight: 9.7 kg

Features:

• Configuration: 48/48-well
• Dual-block Alpha unit
• Capacity: 2 x 48 x 0.2 ml tubes
• Sample Volume: 5–125 µl

Features:
• Configuration: 96-Well
• Single-block Alpha unit
• Capacity: 96 x 0.2 ml tubes, one 96-well plate
• Sample Volume: 5–125 µl

Alpha units are interchangeable reaction modules available in a variety of formats to accommodate standard tubes and plates and high-density microplates.

Every thermal cycler in the DNA Engine family (the DNA Engine, DNA Engine Dyad, Dyad Disciple, and DNA Engine Tetrad 2 cyclers) accepts all interchangeable Alpha units. Changing an Alpha unit takes less than 10 seconds, with no tools required. The temperature accuracy specifications of Alpha blocks ensure consistent results experiment-to-experiment and cycler-to-cycler.

Features:

• Single-block Alpha unit
• Adjustable heated lids set manually; 96-well unit is gradient capable
• Configuration: 96-well
• Capacity: 96 x 0.2 ml tubes, one 96-well plate
• Sample Volume: 5–125 µl
• Catalog: ALS-1296

Includes:

• Cooling Module
• Control Module
• Drive Module

Drive Module 

• Dimensions (W x D x H): 43 x 26 x 14 cm
• Construction: Aluminum chassis
• Weight: 8 kg (17.2 lb)
• Maximum voltage:250 V DC
• Maximum current: 500 mA
• Electrode potentials:Dynamically regulated (feedback adjustment) +/- 0.5%
• Input voltage range:
  • 90–132 VAC/47–63 Hz/0.7 A (maximum)
  • 198–264 VAC/47–63 Hz/0.35 A (maximum)
• Fuses:
  • 5 A Fast Blow for high voltage output (front panel)
  • A Slow-Blow (100/120 V) or
  • 5 A Slow-Blow (220/240 V)

Control Module          

• Dimensions (W x D x H): 31 x 13.5 x 17 cm
• Maximum current: 500 mA
• Allowable voltage gradients:6–6 V/cm, in 0.1 V/cm increments
• Data entry: Keyboard
• Power supply: 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable

Cooling Module

• Chassis: Aluminum
• Dimensions (W x L x H):23 x 42 x 24 cm
• Weight: 14 kg (30.1 lb)
• Compressor:1/12 HP
• Refrigerant:CFC 12
• Heat exchanger:Stainless steel
• Operating range: 5°C–25°C
• Cooling rate (pre-cool):75°C/min from ambient to 14°C
• Cooling capacity (input power): 75 W at 14°C
• Input voltage range       
  • 90–132 VAC/47–63 Hz/4 A
  • 198–264 VAC/47–63 Hz/2 A
• Fuses   
• 3 A Slow-Blow (100/120 V) or
• 0 A Slow-Blow (220/240 V)

Includes:

• Cabinet & Camera
• Computer With Software
• Manual

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

Specifications:

• CCD Resolution (HxV): 1,392 x 1,040µm
• Pixel density: 65,536 gray levels
• Imaging area: 25 x 26 cm
• Camera cooling temperature: -50°C
• Camera cooling system: Peltier
• Dynamic range: 4 orders of magnitude
• Motorized zoom lens: C-mount, f/1.2, 12-75 mm
• Illumination modes: Epi-white, trans-UV (302 nm), and white

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

High quality single-wavelength detector for flowthrough monitoring of effluents from chromatographic columns, centrifugation gradients, and other devices.

Specifications:

• Wavelength: 254 and 280 nm
• Temperature Range: 4° to 40°C
• Detection Limit: 7 µg/ml (BSA in H2O)
• Lamp: Low-Pressure Mercury with Phosphor Screen
• Flow Cell: Optical Path 2 mm
• Internal Volume: 80 µL
• Illuminated Volume: 3 µL
• Noise: 1 x 10^–4 OD max, peak-to-peak (dry cell); 2 x 10^–4 OD max
• Dimensions (Base Units): 5.7” x 17.3” x 8” in. (W x D x H)
• Dimensions (Optics Units): 5” x 6” x 1.5” in. (W x D x H)

Features:

Fragment Size: 100bp-10Mb

Description:

The new Gene Pulser II system is a completely redesigned electroporation system, which offers improved performance, flexibility, sample protection, and safety. The Gene Pulser II system uses the Pulse Trac waveform delivery system to generate the most accurate exponential decay pulses possible for optimal cell transformation within an electroporation cuvette. The Pulse Trac delivery system accurately calculates the time constant of each pulse based on what is actually delivered to the sample.

Specifications:

• Input voltage: 100/120 V RMS, 50/60 Hz
• Input current: 15 amp RMS
• Maximum output voltage and current: 2,500 V at 125 amp peak (normal load); limited to 1,500 A during arc.
• Output waveform: Pulse Trac exponential decay with RC time constant dependent on sample and capacitor selected
• Output voltage adjustment: 50–2,500 V range (depending on the capacitor) with 10 V adjustment precision for the high voltage range and 2 V for the low-voltage range (50–500 V).
• Ambient operating environment: Temperature 0–35°C
• Humidity: 0–95% without condensation
• Regulatory: Passes requirements of IEC 1010. In addition, the system passes requirements for FCC, Class A.
• Dimensions: 34 x 31 x 19 cm
• Weight: 10.41 kg

Includes

• Gene Pulser II
• Pulse Controller Plus
• Capacitance Extender Plus
• Gene Pulser II Shocking Chamber
• 25-pin and 9-pin Interconnect Cables
• Cuvette holder

The GS-800 calibrated densitometer offers superior accuracy, sensitivity, and data reproducibility. It automatically self-calibrates the optical density to optimize detection. Features include transmissive and reflective imaging to scan chromogenic samples at their optimal detection wavelength. It allows for imaging of many different samples such as 1D and 2D gels, colorimetric dot and slot blots, and film based chemiluminescent blots.

Specifications:

• Wavelength:400 - 750 nm
• Absorbance Range:0 - 3.0 OD (transmissive), 0 - 2.0 RD (reflective)
• Scanning Area:Transmissive 29 x 40 cm, reflective 30 x 40 cm
• Sampling Rate:800 x 1600 dpi
• Pixel Density:12 bit
• Dimensions:3 x 62.6 x 19.3 cm (18.6 x 24.6 x 7.6 in)
• Weight: 8 kg (45.9 lb)

384 Well Block

The optical module houses the excitation system and the detection system. The Excitation system consists of a fan-cooled 50 watt tungsten halogen lamp a heat filter (infrared absorbing glass) a 6 position filter wheel fitted with optical filters and opaque filter "blanks" and dual mirror arrangement that allows simultaneous illumination of the entire sample plate.

The excitation system of the BioRad iCycler PCR Thermal Cycler is physically located on the right front corner of the optical module with the lamp shining from right to left perpendicular to the instrument axis. Light originates at the lamp passes through the heat filter and a selected color filter and is then reflected onto the plate in the thermal cycler by a set of mirrors. This light source excites the fluorescent molecules in the wells.

Features:

• Unique modular design for maximum flexibility and sensitivity
• Methods that enable a choice of PCR chemistry
• Outstanding thermal performance from the iCycler thermal cycler
• Gradient functionality for rapid assay optimization
• Easy-to-use software for efficient accurate data analysis
• Compatibility with outstanding PCR supermixes that provide optimum performance in real-time PCR assays

Description:

The MyiQ real time PCR detection system offers an affordable solution for the detection of common fluorescent green dyes such as FAM and SYBR Green I. This system interfaces directly with the iCycler thermal cycler, offering superior features such as thermal gradient capabilities and Peltier-effect driven performance.

Specifications:

• Temperature Range: 4 - 100℃

• Temperature Uniformity: ± 0.4℃

• Heated Lid Temperature: up to 100℃

• Gradient Mode

• Gradient Accuracy: ± 0.4℃

• Row Uniformity: ± 0.4℃

• Gradient Range: 40 - 100℃

• Sample Capacity: 96 Wells

• Sample Size: 15 - 100 µl

• Dimensions (W x D x H): 29.2 x 58.4 x 38.7 cm

• Fluorescence Excitation Range: 475 - 495 nm

• Fluorescence Detection Range: 515 - 545 nm

• Dynamic Range: 9 orders of magnitude