Used Lab Equipment

Features:

• Configuration: 48/48-well
• Dual-block Alpha unit
• Capacity: 2 x 48 x 0.2 ml tubes
• Sample Volume: 5–125 µl

Alpha units are interchangeable reaction modules available in a variety of formats to accommodate standard tubes and plates and high-density microplates.

Every thermal cycler in the DNA Engine family (the DNA Engine, DNA Engine Dyad, Dyad Disciple, and DNA Engine Tetrad 2 cyclers) accepts all interchangeable Alpha units. Changing an Alpha unit takes less than 10 seconds, with no tools required. The temperature accuracy specifications of Alpha blocks ensure consistent results experiment-to-experiment and cycler-to-cycler.

Features:

• Single-block Alpha unit
• Adjustable heated lids set manually; 96-well unit is gradient capable
• Configuration: 96-well
• Capacity: 96 x 0.2 ml tubes, one 96-well plate
• Sample Volume: 5–125 µl
• Catalog: ALS-1296

CHEF technology yields straight lanes over a wide range of DNA molecule sizes. The CHEF-DR^® II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.

The CHEF-DR II system effectively resolves DNA fragments in the range of 5 kb to 6 Mb. This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system exclusively employs the most common angle of electrophoresis for PFGE, 120°. This system is simple to program, and has proven to be a solid performer. Each CHEF-DR II system is supplied with an electrophoresis cell, drive module, control module, variable-speed pump, and accessory kit. The CHEF-DR II chiller systems also come with a cooling module.

Includes:

• Cooling Module
• Control Module
• Drive Module

Drive Module 

• Dimensions (W x D x H): 43 x 26 x 14 cm
• Construction: Aluminum chassis
• Weight: 8 kg (17.2 lb)
• Maximum voltage:250 V DC
• Maximum current: 500 mA
• Electrode potentials:Dynamically regulated (feedback adjustment) +/- 0.5%
• Input voltage range:
  • 90–132 VAC/47–63 Hz/0.7 A (maximum)
  • 198–264 VAC/47–63 Hz/0.35 A (maximum)
• Fuses:
  • 5 A Fast Blow for high voltage output (front panel)
  • A Slow-Blow (100/120 V) or
  • 5 A Slow-Blow (220/240 V)

Control Module          

• Dimensions (W x D x H): 31 x 13.5 x 17 cm
• Maximum current: 500 mA
• Allowable voltage gradients:6–6 V/cm, in 0.1 V/cm increments
• Data entry: Keyboard
• Power supply: 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable

Cooling Module

• Chassis: Aluminum
• Dimensions (W x L x H):23 x 42 x 24 cm
• Weight: 14 kg (30.1 lb)
• Compressor:1/12 HP
• Refrigerant:CFC 12
• Heat exchanger:Stainless steel
• Operating range: 5°C–25°C
• Cooling rate (pre-cool):75°C/min from ambient to 14°C
• Cooling capacity (input power): 75 W at 14°C
• Input voltage range       
  • 90–132 VAC/47–63 Hz/4 A
  • 198–264 VAC/47–63 Hz/2 A
• Fuses   
• 3 A Slow-Blow (100/120 V) or
• 0 A Slow-Blow (220/240 V)

Includes:

• Cabinet & Camera
• Computer With Software
• Manual

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

Specifications:

• CCD Resolution (HxV): 1,392 x 1,040µm
• Pixel density: 65,536 gray levels
• Imaging area: 25 x 26 cm
• Camera cooling temperature: -50°C
• Camera cooling system: Peltier
• Dynamic range: 4 orders of magnitude
• Motorized zoom lens: C-mount, f/1.2, 12-75 mm
• Illumination modes: Epi-white, trans-UV (302 nm), and white

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

Features:

Fragment Size: 100bp-10Mb

Specifications:

• Timer control:
• Dimensions: 0-3 hr, fully adjustable
• Weight: 18 lbs (8 kg)
• Inner dimensions: 20 x 20 cm
• Gel capacity: 4 mini(8 x 7cm) gels, 2 criterion (13.3 x 8.7cm) gels, 1 large (20 x 20cm) gel (per frame)

Description:

The new Gene Pulser II system is a completely redesigned electroporation system, which offers improved performance, flexibility, sample protection, and safety. The Gene Pulser II system uses the Pulse Trac waveform delivery system to generate the most accurate exponential decay pulses possible for optimal cell transformation within an electroporation cuvette. The Pulse Trac delivery system accurately calculates the time constant of each pulse based on what is actually delivered to the sample.

Specifications:

• Input voltage: 100/120 V RMS, 50/60 Hz
• Input current: 15 amp RMS
• Maximum output voltage and current: 2,500 V at 125 amp peak (normal load); limited to 1,500 A during arc.
• Output waveform: Pulse Trac exponential decay with RC time constant dependent on sample and capacitor selected
• Output voltage adjustment: 50–2,500 V range (depending on the capacitor) with 10 V adjustment precision for the high voltage range and 2 V for the low-voltage range (50–500 V).
• Ambient operating environment: Temperature 0–35°C
• Humidity: 0–95% without condensation
• Regulatory: Passes requirements of IEC 1010. In addition, the system passes requirements for FCC, Class A.
• Dimensions: 34 x 31 x 19 cm
• Weight: 10.41 kg

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus

The GS-800 calibrated densitometer offers superior accuracy, sensitivity, and data reproducibility. It automatically self-calibrates the optical density to optimize detection.Unlike laser densitometer systems, the GS-800 calibrated densitometer has both transmittance and true reflectance capabilities that allow accurate scans of both transparent and opaque samples. The advantage of true reflectance scanning is accurate detection and analysis of molecules on the surface of membranes, rather than molecules distributed throughout the membrane matrix.

Specifications:

• Wavelength: 400-750 nm
• Absorbance Range:0 - 3 OD (transmissive), 0 - 2 OD (reflective)
• Scanning Area: 29 x 40 cm (transmissive), 30 x 40 cm (reflective)
• Sampling Rate:800 x 1600 dpi
• Pixel Density:12 bit
• Dimensions: 3 x 62.6 x 19.3 cm
• Weight:8 kg

384 Well Block

The optical module houses the excitation system and the detection system. The Excitation system consists of a fan-cooled 50 watt tungsten halogen lamp a heat filter (infrared absorbing glass) a 6 position filter wheel fitted with optical filters and opaque filter "blanks" and dual mirror arrangement that allows simultaneous illumination of the entire sample plate.

The excitation system of the BioRad iCycler PCR Thermal Cycler is physically located on the right front corner of the optical module with the lamp shining from right to left perpendicular to the instrument axis. Light originates at the lamp passes through the heat filter and a selected color filter and is then reflected onto the plate in the thermal cycler by a set of mirrors. This light source excites the fluorescent molecules in the wells.

Features:

• Unique modular design for maximum flexibility and sensitivity
• Methods that enable a choice of PCR chemistry
• Outstanding thermal performance from the iCycler thermal cycler
• Gradient functionality for rapid assay optimization
• Easy-to-use software for efficient accurate data analysis
• Compatibility with outstanding PCR supermixes that provide optimum performance in real-time PCR assays

Description:

The MyiQ real time PCR detection system offers an affordable solution for the detection of common fluorescent green dyes such as FAM and SYBR Green I. This system interfaces directly with the iCycler thermal cycler, offering superior features such as thermal gradient capabilities and Peltier-effect driven performance.

Specifications:

• Temperature Range: 4 - 100℃

• Temperature Uniformity: ± 0.4℃

• Heated Lid Temperature: up to 100℃

• Gradient Mode

• Gradient Accuracy: ± 0.4℃

• Row Uniformity: ± 0.4℃

• Gradient Range: 40 - 100℃

• Sample Capacity: 96 Wells

• Sample Size: 15 - 100 µl

• Dimensions (W x D x H): 29.2 x 58.4 x 38.7 cm

• Fluorescence Excitation Range: 475 - 495 nm

• Fluorescence Detection Range: 515 - 545 nm

• Dynamic Range: 9 orders of magnitude

Bio-Rad Power Pac 300 is an electrophoresis power supply that can provide voltages from 10 to 300V. It has a current output range from 4 to 400mA and has a maximum power output of 75W. It can connect up to 4 electrophoresis cells in parallel.

Specifications:

• Max Voltage: 300V
• Max Current: 400mA
• Max Wattage: 75W

Features:

• Large 12 cm (4.7”) high-resolution display
• Programming using intuitive symbols and numerical entry procedures
• Graphical representation of protocols
• Menu-driven software and ready-made templates for ease of viewing, editing, and running protocols
• Innovative thermal gradient design (optional upgrade)
• Reaction module and self-adjusting lid accommodate 96 x 0.2 ml samples in tubes, strips, or plates
• Power-saving programmable automatic standby mode
• USB communication for printing of protocols and reports
• Storage of detailed run and validation reports
• Attractive, compact design

Specifications:

• Heating rate: Up to 2.5°C/sec
• Cooling rate: Up to 1.5°C/sec
• Method of heating/cooling: Peltier
• Modes of temperature monitoring: Block and algorithm
• Temperature Range: 4–100°C
• Temperature Accuracy: ±0.5°C
• Temperature Uniformity: ±0.5°C
• Overshoot maximum: 0.8°C
• Heated lid: Up to 110°C
• Gradient accuracy: ±0.5°C of programmed target at 90°C
• Row uniformity: ±0.5°C well-to-well within 30 sec of reaching target temperature
• Gradient range: 30–99°C
• Temperature differential range: 1–25°C
• Descriptive Sample capacity: 96 x 0.2 ml tubes or 96-well PCR plate
• Display: 12 cm (4.7”) high-resolution
• Operating temperature: 18 – 28°C ambient
• Ports: 1 USB
• Electrical approvals: IEC, CE
• Dimensions (W x D x H): 24 x 44 x 21 cm (9 x 17.5 x 8”)
• Weight: 10 kg (22 lb)
• Programming Memory: 99 typical programs
• Cycles per protocol: Up to 9
• Segments per cycle: Up to 9
• Repeats per cycle: Up to 99
• Segment time range: 00:01–99:59 min