Used Lab Equipment

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

• Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
• Increased sample throughput with greater efficiency.
• Reduced sample volume results in less reagent use.
• Auto focus capability increases instrument ease of use.
• Variable aspiration and mixing cycles optimize results.
• Enhanced image magnification provides unparalleled cellular detail.
• Circularity measurement helps isolate debris from cells.

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

Features:

The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells.

Specifications:

• Cell Number per Assay: User defined; cell concentrations of 10,000–500,000 cells/mL
• Sample Format: Single loader
• Sample Volume: 200 µL
• Emission Max: Yellow: 28nm bandwidth at 576nm, Red: 30nm bandwidth at 680nm, Forward scatter = laser wavelength
• Excitation Max: Green Laser (532nm)
• Scatter: Forward
• Throughput: Less than 2 minutes per sample
• Dimensions 8.69” H  x 8.12” W x 11.11” L

"Fully automated and easy-to-use, the nCounter MAX Analysis System provides everything you need to cost-effectively complete your projects in record time. More accurate and highly multiplexed than qPCR and simpler than NGS, the nCounter MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands on time. Accelerate your research by spending less time on sample prep and perform your own data analysis using the included nSolver Analysis Software.

Specification:

Level of multiplexing: Up to 800 targets

Recommended amount of starting material (dependent on assay and sample type)
RNA: 1 ng - 50 ng
DNA 5 ng - 300 ng
Protein: 250 ng - 2.5 µg
Sample types supported: Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene-lysed whole blood
Reaction volume: up to 30 µg
Limit of detection 0.5 fM spike-in control: 15 zeptomole spike-in control

Fold change sensitivity
>1.5-fold (if > 5 copies per cell)
> 2-fold (if > 1 copy per cell)
Spike correlation: R² ≥ 0.95
Linear dynamic range: 6 x 10⁵ total counts
Prep Station throughput: 12 lanes per 2.5 hours
Digital Analyzer throughput: 12 samples per 2.7 hours
Controls: Assay dependent
Operating temperature: 18–28°C
Humidity: 30-80% (non-condensing)
Pollution degree: 2
Power source: 30-80% (non-condensing)
Power draw: 610 VA
Dimensions (Prep Station): 67 x 89 x 63 cm
Dimensions (Digital Analyzer): 66 x 66 x 48 cm
Weight (Prep Station): 120 kg
Weight (Digital Analyzer): 67 kg"

This compact system is equipped with one laser, two fluorescence detectors and forward and side-scatter detectors.

Features of the S3e 488 Cell Sorter (one-laser):

• Hands-free nozzle tip alignment features the fully automatic AutoGimbal System, driven completely by software
• Simplified instrument setup fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 14 color sorting with minimal training
• 2-way cell sorting sort two different defined populations at the same time
• High speed and high purity sort cells fast while maintaining high sensitivity and purity
• Intuitive interface user-friendly ProSort Software enables effortless instrument control and sort logic definition

Manufacture Specification

• Droplet frequency: 3743 kHz
• Sorting type: True jet-in-air for high-performance sorting
• Sorting rate: No hardware limitations for sort rate, limited only by droplet frequency and application
• Sorting purity: >99% pure
• Nozzle size: 100 m
• Sorting direction: 2-way sorting
• Sorting collection: Up to 5 x 5 ml sample tubes each direction
• Up to 5 x 1.5 ml tubes each direction
• Microscope slides
• 8-well strip each direction
• Lasers: Single-laser: 488 nm 100 mW
• Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
• Dual-laser: 488 and 561 or 640 nm 100 mW
• Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW
• Detection: Forward scatter (FSC) with PMT
• Side scatter (SSC) with PMT
• Up to 4 fluorescence detectors with PMT: Minimum resolution: 0.5 m
• Sensitivity: <125 MESF for FITC and PE
• Temperature range: Sample and collection temperature control system: 437C
• Peltier solid state system
• Fluidics: Onboard fluidics and dilution of 8x sheath fluid with deionized water
• Data format: FCS 3.1

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Features:

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

Specifications:

• User-friendly, benchtop design for simple, fast, automated cell count and viability measurements within a minute  
• Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes  
• Measures cell concentrations ranging from 1 × 104 to 1 × 107 cells/mL and cells with sizes ranging from 5 μm to 60 μm 
• Uses disposable counting chamber slides that eliminate washing steps and cross contamination between samples  
• Saves and prints cell count data including images using the Countess™ software and Countess™ USB drive  
• Presents comprehensive data with graphical reports and as a .CSV (comma separated value) file for sample comparisons  
• Allows you to gate and analyze cells based on cell size 

Specifications:

• Capacity: One 2-chamber disposable or 2-chamber reusable slide
• Cell Size: 4-60 µm (detection), 7-60 µm (viability)
• Concentration: 1x10^4-1x10^7 cells⁄mL
• Detection Method: Brightfield & 2 user-definable FL channels
• Dimensions: 9 in (228.6 mm) W x 5.5 in (139.7 mm) D x 9 in (228.6 mm) W
• Display: 7in Capacitive touch screen
• Field: 2.15 mm x 1.62 mm (3.48 mm2)
• Voltage: 100-240 VAC
• Weight (English): 8 lbs
• Memory: USB Stick

Specifications:

• Capacity: One 2-chamber disposable or 2-chamber reusable slide
• Cell Size: 4-60 µm (detection), 7-60 µm (viability)
• Concentration: 1x10^4-1x10^7 cells⁄mL
• Detection Method: Brightfield & 2 user-definable FL channels
• Dimensions: 9 in (228.6 mm) W x 5.5 in (139.7 mm) D x 9 in (228.6 mm) W
• Display: 7in Capacitive touch screen
• Field: 2.15 mm x 1.62 mm (3.48 mm2)
• Voltage: 100-240 VAC
• Weight (English): 8 lbs
• Memory: USB Stick

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Features:

The FastPrep® FP120 is a high-speed, benchtop reciprocating device for efficient
disruption of cell membranes. The unit is designed for use with the FastPrep®
Extraction Kits (Qbiogene, www.qbiogene.com). These kits are available for a
wide variety of extraction and purification applications.

Specifications:

• Controls: Programmable run time and speed; large LED readout
• Time: Range: 1-45 sec/ Programmable in 1 sec increments
• Speed: Range: 4.0-6.5m/sec/ Programmable in 0.5 m/sec increments
• Acceleration: < 2 seconds to maximum speed
• Deceleration: < 2 seconds to maximum speed
• Dimensions (W*D*H): 11 in x 15.75 in x 12 in

Specifications:

• Loading time: App. 60 ul
• Measurement volume: 1.4 ul
• Analysis time: 50 sec (one step), 120 sec (two step)
• Dimensions: 38 x 26 x 22 cm (W x H x D)
• Weight: 6.7 lb (3 kg)