Used Lab Equipment

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

• Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
• Increased sample throughput with greater efficiency.
• Reduced sample volume results in less reagent use.
• Auto focus capability increases instrument ease of use.
• Variable aspiration and mixing cycles optimize results.
• Enhanced image magnification provides unparalleled cellular detail.
• Circularity measurement helps isolate debris from cells.

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Specifications:

• Volume: 5-100 mL

Description:

• Bottle Positions: 10   
• Dimensions (L x D x H): 73 cm x 32 cm x 37 cm
• Bottle Speed: .01 To 4 rpm    
• Clearance Between Decks For Bottle Placement: 134 mm   
• Usable Roller Length: 28 cm   
• Electrical Rating: 115 +/- 10 VAC @ 60 Hz Line Voltage 1.0 Amp @ 115 V, 60 Hz Power Consumption   
• Environmental Conditions: Indoor Use, Pollution Degree 24-50 Degree C, Relative Humidity 0-90%, Non-condensing
• Fits inside bellco benchtop incubators  
• Accepts bellco glass & plastic roller bottles both 110 & 120 mm diameter up to 28 cm overall length   
• All Position Drive   
• Variable Speed Range    
• Analog Tachometer
• Cat#: 7630-S0019
• 9 decks, 5 spots per deck

Description:

These cost-effective vertical laminar flow and horizontal laminar flow workstations feature the Clean-Ceil fan filter module, which utilizes a HEPA filter that is 99.99% efficient for all particles >=0.3µm. The workstations may be used as either benchtop or console units. They incorporate a modular design approach that allows the user to customize a workstation by choosing the optional accessories necessary to meet individual requirements. The workstations come complete with aluminum support frame, clear Plexiglas side panels, 40% efficient ASHRAE-rated prefilter, white plastic laminate back panel (vertical model only, filter is on top), or clear Plexiglas top panel (horizontal model only, filter is on back).

Specifications:

• Dimensions (D x H x W): 21” x 22” x 46”
• Electrical: 115 V, 60 Hz
• Air velocity: 90 ±20 fpm

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

The FastPrep FP120 Cell Disrupter can simultaneously lyse up to 12 samples with its patented vertical, angular motion. This rapid, reciprocating "figure-8" is unique to the FastPrep Instrument, and causes a sample to be pulverized by specialized lysing matrices from multiple directions at once.The FastPrep System combines two products into one: A multi-tube homogenizer and a molecular isolation kit. The FastPrep is unique system for isolating DNA, RNA and protein from virtually any type of cell.

Specifications:

• Time: Range: 1-45 sec, programmable in 1 sec increments
• Speed: Range: 4.0-6.5m/sec, programmable in 0.5 m/sec increments
• Acceleration: < 2 seconds to maximum speed
• Deceleration: < 2 seconds to stop
• Duty Cycle: 6.5 m/s for 45 seconds with a 5-minute rest period between runs
• Dimensions: 11 x 16 x 13 in
• Power Requirements: 115V, 50/60Hz, 8A

The PROTEAN IEF system enables high-throughput two-dimensional (2-D) protein separation. Consisting of the PROTEAN IEF cell and ReadyStrip IPG strips for isoelectric focusing (IEF) of proteins in 2-D applications, this unique system offers major benefits: streamlined handling, enhanced resolution, and increased reproducibility. Whether you run 10 gels a month or 10 gels a day, the PROTEAN IEF system consistently provides great 2-D results. The IEF cell is part of the Expression Proteomics workflow, which covers everything from sample preparation to spot excision.

Features/Specifications

• High-Capacity platform holds 1 large or 2 mini trays
• Allows up to 24 immobilized pH gradient strips per run
• 3 preprogrammed methods enable quick start to electrophoresis
• Real-time editing capability gives maximum control over the run
• Peltier-cooled platform maintains consistent temperature for reproducibility
• Integral 10,000v power supply allows you to reach the desired volt-hour target faster

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Configuration:

• Holds Up to 24 Tubes/Vials for 2700 System
• Sample Container Diameter: 10-16 mm
• Sample Container Height: 20 to 100 mm