Used Lab Equipment

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

The Trans-Blot Turbo transfer system reduces western blotting setup time to 1 minute and transfer to as little as 3 without sacrificing sensitivity.

Paired with Trans-Blot Turbo transfer packs or Trans-Blot RTA kits, this system provides a complete solution for fast, high-efficiency transfer of even high molecular weight proteins.
Used on its own or as part of the V3 Western Workflow, the Trans-Blot Turbo system is the no-compromise solution for high-performance western blotting.

Features:

• Fast Transfer Times: Transfer your proteins in as little as 3 minutes.
• High Throughput: Transfer up to 4 mini or 2 midi gels simultaneously.
• Flexible Design: Easily customize transfer conditions; compatible with traditional semi-dry consumables.
• Ease of Use: Reduce set-up time to only 1 minute from opening the gel cassette to starting the transfer.
• Two Blotting Sandwich Options: Trans-Blot Turbo Transfer Packs or Trans-Blot Ready-to-Assemble Kits

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Description:

Boekel Scientific offers a wide variety of Dry Bath Incubators designed to accommodate an assortment of interchangeable Heating Block Modules. These reliable and economical products are ideal for a variety of applications including restriction digests, C.O.D. denaturing DNA, BUN, melting agar, coagulation studies, in situ hybridization and Hot Start PCR.

Specification:

• Temperature Range: +5ºC to 120ºC
• Stability: ±0.2°C @ 37°C

The Fisher Scientific Dry Bath Incubators offer exceptional temperature control and uniformity in addition to a powder coated steel body to provide durability for demanding applications.

Specifications:

• Temperature Range: 5 - 130 °C
• Temperature Stability: +/-1.5 °C
• Temperature Uniformity: +/-0.5 °C
• Power Requirements: 120V, 50/60Hz

Provide controlled dry heat for a wide variety of clinical and general chemistry applications.

Specifications:

• Temperature Range: 5°C - 130°C
• Power Requirements: 120V, 50/60Hz

The Fisher Scientific dry bath incubator 11-718-2 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C

The Fisher Scientific dry bath incubator 11-718-2 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C

The Fisher Scientific dry bath incubator 11-718-4 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C

Features

• Hydraulic thermostat
• Blocks are interchangeable with digital models

Specifications:

• Temperature Range: Ambient +5 °C to 150 °C
• Temperature Uniformity: ±0.50 °C at 37 °C
• Temperature Stability: ±0.45 °C at 37 °C
• Power Requirements: 115 VAC, 100 W
• Dimensions (W x D x H): 8 x 9.8 x 3.5 in

Boekel Digital Dry Bath Incubators are designed to be used with standard aluminum dry blocks (nominally 3” x 3¾” x 2” high) of varying configurations for heating test tubes, microtubes and other small vessels. These Incubators are available in two sizes; Model 113002 has a two block capacity and includes an easily programmed PID (proportional integrative derivative) controller for rapid heat-up and accurate temperature control.

Specification:

• Dimensions (W x D x H): 8 x 9.8 x 3.5 in. (20.3 x 24.9 x 8.6 cm)
• Net weight: 5 lbs. (2.3 kg)
• Power: 115 VAC, 200 watts / 230 VAC, 230 watts
• Temperature range: ambient 5°C to 120°C
• Uniformity: ±0.25°C at 37°C
• Stability: ±0.2°C from 37 - 100°C

The Fisher Scientific 125D heat block accommodates 2 blocks and features high wattage, constant-temperature heaters are economical, versatile, and compact. This model accepts several interchangeable blocks, accommodating various tube sizes.

Specifications:

• Temperature Range: 5° to 130°C
• Temperature Uniformity: ±0.4° at 37°C
• Dimensions: 10 x 8 x 9 in
• Power Requirements: 115V, 50/60Hz, 2A

VWR Digital Dry Block Heaters are ideal for incubation and activation of cultures, enzyme reactions, blood urea nitrogen determinations, immunoassays, melting/boiling points, and a wide variety of other lab procedures.

Features:

• Environmentally Preferable Attribute
• Exceptional temperature uniformity and accuracy (±0.1°C)
• Hold interchangeable, modular heating blocks
• Heat to 120°C (248°F)
• Optional external temperature probe
• LED display