Used Lab Equipment

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Description:

Boekel Scientific offers a wide variety of Dry Bath Incubators designed to accommodate an assortment of interchangeable Heating Block Modules. These reliable and economical products are ideal for a variety of applications including restriction digests, C.O.D. denaturing DNA, BUN, melting agar, coagulation studies, in situ hybridization and Hot Start PCR.

Specification:

• Temperature Range: +5ºC to 120ºC
• Stability: ±0.2°C @ 37°C

The Fisher Scientific Dry Bath Incubators offer exceptional temperature control and uniformity in addition to a powder coated steel body to provide durability for demanding applications.

Specifications:

• Temperature Range: 5 - 130 °C
• Temperature Stability: +/-1.5 °C
• Temperature Uniformity: +/-0.5 °C
• Power Requirements: 120V, 50/60Hz

This LCMS brings you the best in quantitative sensitivity and robustness to expand upon the experiments going beyond MRM, and give you substantial improvements in data quality.

Includes:

• AB Sciex 4500 LC/MS/MS
• Shimadzu Prominence 20 UFLC  
• Shimadzu DGU-20A3 Degasser
• (2)Shimadzu LC_20AD Liquid Chromatograph
• Shimadzu CTO-20AC Column Oven
• Shimadzu SIL-20AC UFLC
• Windows 7 Computer Loaded with Analyst 1.6 Software       

Specifications:

• Sensitivity MRM: mode Reserpine 200 fg on column S/N > 2000  C.V. < 5%
• Maximum scan speed: 12,000 Da/sec
• Polarity switching time: 50 msec, in MRM and scheduled MRM (sMRM) modes
• Minimum MRM dwell time: 1 msec
• Mass range (m/z): 5-2000
• Cross talk for reserpine 609/195: <0.5% cross talk detectable with 2 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion MS for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), and scheduled MRM (sMRM)
• Detector type: AcQuRate Pulse Counting CEM
• Dynamic range: 4 orders of magnitude
• Ionization sources: Turbo V source housing with TurboIonSpray probe or APCI probe.
• Maximum temperature: 750ºC
• Flow rate compatibility: 5 μL/minute to 3 mL/minute
• Power: 208V 50/60Hz AB Sciex 4500, 208V Vacuum Pump, 208V Peak N2 Generator, 115V Shimadzu UPLC
• Dimensions: 37" x 33" x 55" Sciex 4500
• Weight: 250lbs

 Includes One Year Service Contract, PM at 6 Months, and Installation

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

Includes:

• AB Sciex 5500 Qtrap LC/MS/MS
• Agilent 1200 HPLC System
  
  • Agilent G1322A Degasser
  • Agilent G1311A Quat Pump
  • Agilent G1315D DAD (Diode Array Detector)
• Solvent Tray
• Computer Loaded with Analyst 1.6 Software       

Specifications

• Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
• V.: < 5%
• Triple quad scan speed: 12,000 Da/sec
• Linear ion trap scan speed: 20,000 Da/sec
• Polarity switching time: 50 msec
• Minimum MRM Dwell Time: 1 msec
• Triple quad mass range (m/z): 5-1250
• Linear ion trap mass range (m/z): 50-1000
• Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap scan modes

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus

Provide controlled dry heat for a wide variety of clinical and general chemistry applications.

Specifications:

• Temperature Range: 5°C - 130°C
• Power Requirements: 120V, 50/60Hz

The Fisher Scientific dry bath incubator 11-718-2 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C

The Fisher Scientific dry bath incubator 11-718-2 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C

The Fisher Scientific dry bath incubator 11-718-4 features low and high temperature settings with exceptional temperature control and uniformity. The power coated steel body construction provides durability for demanding applications and the compact design saves valuable bench space.

Specifications:

• Power Requirements: 115V, 2.3A, 50/60Hz
• Temperature Range: 5°C above ambient to 130°C