Used Lab Equipment

The FLUOstar Omega is a multifunctional microplate reader that supports a wide variety of applications and the following reading modes: fluorescence intensity, time-resolved fluorescence, absorbance and luminescence.

Specification:

• Measurement principles

• Fluorescence intensity
• Time-resolved fluorescence -TR-FRET (opt.)
• Luminescence (opt.)
• UV/VIS absorbance spectra
• AlphaScreen (opt.)
• Light source: High-energy xenon flash-lamp
• Detector: side window photomultiplier tube
• Filters: 2 filter wheels with 8 excitation and 8 emission filter positions
• Gain control: Software selectable gain, automatic gain adjustment

• Plate carrier

• Auto lock microplate carrier
• All microplate formats up to 1536-well
• Microplates should fulfill the SBS specification and non-SBS formats
• Should fit (L x W x H): 128 x 86 x 22 mm; min. length 124

• Regent injector

• Up to two built-in reagent injectors
• Individual injection volumes for each well
• Injection volumes definable down to 3µl
• Up to 4 independent injection actions per well
• Variable injection speed (100µl/s to 420µl/s)

• Shaking

• Linear, orbital and figure eight shaking
• Programmable speed and shake time

• Incubation

• Incubation range from ambient +5°C to 45°C, in 0.1°C steps
• Extended incubation up to 60°C (optional)
• Temperature monitoring (without incubation)
• Temperature stability 0.2°C

• Fluorescence intensity

• Limit of detection 0.2 fmol / well fluorescein
• Spectral range (ex. and em.): 240 … 740 nm (900 nm opt.)
• Dynamic range 8 decades

• Time-resolved fluorescence

• Limit of detection: 30 amol / well europium
• Advanced TRF/TR-FRET: 3 amol / well europium
• Spectral range (ex. and em.): 240 ... 740 nm (900 nm opt.)
• Dynamic range: 6 decades

• Luminescence

• Limit of detection 20 amol / well ATP
• Spectral range 240 ... 740 nm
• Dynamic range: 9 decades

• Fluorescence polarization

• Limit of detection: <5 mP SD at 1 nM fluorescein
• Spectral range: (ex. and em.): 380 ... 740 nm (900 nm opt.)
• Dynamic range: 4 decades

• Absorbance Spectrophotometer based

• Spectral range 220 ... 850 nm
• Full spectrum captured in < 1 s/well
• Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
• OD range: 0 to 4 OD
• Accuracy: < 1% at 2 OD
• Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
• Computer interface: USB 2.0, compatible to USB 1.1
• Dimensions (H x W x L): 31 x 44 x 48 cm
• Weight: 29 kg

• Ambient conditions

• Operating temperature: 15°C to 35°C
• Storage temperature: -10°C to 50°C
• Humidity of atmosphere: 20% to 80% (Non-condensing)
• Instrument conformity: Overvoltage category II; contamination class II; protection class I
• Robotic capabilities: stacker for 50 microplates (optional)

The Gemini XPS Fluorescence Microplate Reader uses dual monochromators for variable wavelength selection between 250 nm and 850 nm to eliminate the need for searching out the right pair of excitation and emission filters. Dual monochromators also enable wavelength scanning across a range of wavelengths in increments as small as 1 nm to optimize assay parameters.

Includes:

• Computer loaded with Softmax Pro
• All Necessary Cables and Cords
• Manuals

General Specifications

• Dimensions (in.): 8.6 (h) x 22.8 (w) x 15 (d)
• Dimensions (cm): 22 (h) x 58 (w) x 38 (d)
• Weight: 35 lb (16 kg)
• Power source: 100-240 VAC, 50/60 Hz

Performance Specifications

• Wavelength Range - Excitation: 250-850 nm
• Wavelength Range - Emission: 360-850 nm
• Wavelength Selection: Monochromator, tunable 1.0 nm increments
• Wavelength Bandwidth (Excitation and Emission): 9 nm
• Wavelength Accuracy: < ± 2.0 nm
• Top Read Sensitivity: 3.0 fmol/well FITC 200 µl in 96 wells
• Light Source: Xenon flash lamp
• Microplate Read Time:
  • Normal Mode
  • 96 wells. . . . 15 seconds
  • 384 wells. . . . 45 seconds
• Dynamic Range: 6 orders of magnitude
• Temperature Range: Ambient + 4 °C up to 45 °C
• Temperature Uniformity (Microplate): ± 1 °C @ 37 °C

The Gemini XPS Microplate Reader supports a multitude of fluorescence assay categories including:

• ELISAs and Immunoassays
• Nucleic Acid (DNA) Quantitation
• Protein Quantitation
• Reporter Gene Assays
• Cell Viability, Proliferation, and Cytotoxicity
• Enzyme Assays
• Transporter Assays
• Phosphotases/Kinases
• Microbial Growth