Used Lab Equipment

Includes:

• Computer Loaded with SoftMax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

The SPECTRAmax 250 microplate spectrophotometer has a xenon flash lamp and grating monochromator that facilitate direct UV measurement of DNA- RNA- and protein in microplates. Up to 96 assays can be run at one time using disposable or quartz microplates. The purity of DNA can be measured with A260/A280 ratios. Also- users can perform spectral scans from 250 nm to 750 nm to simplify qualitative analyses of numerous samples. The product reportedly reduces sample volumes in experiments because the assays are performed in a microplate instead of cuvettes. SOFTmax PRO microplate data- analysis software- for use with the SPECTRAmax 250- provides built-in spreadsheet capabilities for easy data analysis and reporting.

Specifications:

• Wavelength Range: 250-700 nm
• Photometric Range: 0.000 to 4.000 OD
• Wavelength Selection: Monochromator, tunable in 1-nm increments
• Photometric Linearity (405 nm): 0 to 3.000 OD
• Wavelength Bandwidth: 5 nm
• OD Resolution: 0.001 OD
• Endpoint Read Time: 9 seconds
• Temperature Range: Ambient to 45 C
• Kinetic Read Time: 9-second minimum interval
• Temperature Uniformity (Microplate): +/-0.5 C @ 37 C well-to-well

Includes:

• Computer Loaded with Softmax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

General Specifications

• Dimensions (in.): 8.6 (h) x 22.8 (w) x 15 (d)

Performance Specifications

• Wavelength Range: 340 - 850 nm
• Wavelength Selection: Monochromator, tunable 1.0 nm increments
• Wavelength Bandwidth: 2 nm
• Wavelength Accuracy: < ± 1.0 nm
• Wavelength Repeatability: ± 0.2 nm
• Photometric Range: 0.0 to 4.0 OD
• Photometric Accuracy: < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
• Photometric Precision: < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
• Light Source: Xenon flash lamp

• Temperature Range: Ambient + 4 °C up to 45 °C
• Temperature Uniformity: ± 0.5 °C @ 37 °C

Microplate Read Time:

• Endpoint
• 96-well: 9 seconds
• 384-well: 29 seconds
• Kinetic
• 96-well: 9 second minimum interval
• 384-well: 29 second minimum interval

Premier dual-mode readers for absorbance & fluorescence intensity-based assays. Users can select from top- or bottom-read modes for improved sensitivity for solution and cell-based assays.

Includes:

• SoftMax Pro software version 5.4.1
• Computer Workstation w/ Flat Screen Monitor
• Complete Set of Communication & Power Cables
• User/Reference Manuals for M2 and SoftMax Pro software

Specifications:

• Absorbance Photometric Performance
• Wavelength range: 200-1000 nm
• Wavelength selection: Monochromator
• Wavelength bandwidth: ≤ 4.0 nm
• Wavelength accuracy: ±2.0 nm
• Wavelength repeatability: ±0.2 nm

Fluorescence Photometric Performance (M2)

• Dual monochromators: 1 nm increments
• EX 250-850 nm
• EM 360-850 nm
• Bandwidth (EX, EM): 9, 9 nm
• Detection limit: 3.0 fmol/well FITC 200 μL in 96 wells

Premier dual-mode readers for absorbance & fluorescence intensity-based assays. Users can select from top- or bottom-read modes for improved sensitivity for solution and cell-based assays.

Includes:

• SoftMax Pro software version 5.4.1
• Computer Workstation w/ Flat Screen Monitor
• Complete Set of Communication & Power Cables
• User/Reference Manuals for M2 and SoftMax Pro software

Specifications:

• Absorbance Photometric Performance
• Wavelength range: 200-1000 nm
• Wavelength selection: Monochromator
• Wavelength bandwidth: ≤ 4.0 nm
• Wavelength accuracy: ±2.0 nm
• Wavelength repeatability: ±0.2 nm

Fluorescence Photometric Performance (M2)

• Dual monochromators: 1 nm increments
• EX 250-850 nm
• EM 360-850 nm
• Bandwidth (EX, EM): 9, 9 nm
• Detection limit: 3.0 fmol/well FITC 200 μL in 96 wells

The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates.

Includes:

• Computer w/ Softmax Pro 5.4
• Manuals
• Cables

Multiple detection modes

• UV-Visible Absorbance (Abs)
• Fluorescence Intensity (FI)
• Time-Resolved Fluorescence (TRF)
• Fluorescence Polarization (FP)
• Glow Luminescence (Lumi)

Features

• Multiple Detection Modes
• Precision Optics and Detectors
• Patented AutoPMT Optimization System
• Patented PathCheck® Pathlength Measurement Technology
• Comprehensive Reader Validation
• Automating with Ease

General Photometric performance

Plate Formats

• 6, 12, 24, 48, 96, 384 wells

Light Source

• Xenon Flash Lamp (1 joule/flash)

Detectors

• 2 photomultiplier tubes

ShakerTime

• 0 to 999 seconds

Temperature Control

• 2 °C above ambient to 60 °C

Temperature Uniformity

• < 1°C at 37°C set point

Temperature Accuracy

• ±1°C at 37°C set point

Endpoint Reading

• All modes

Kinetic Reading

• All modes

Spectral Scanning

• All modes

Well Scanning

• Abs, FI, TRF, Lum

The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates.

Includes:

• Computer w/ Softmax Pro 5.4
• Manuals
• Cables

Multiple detection modes

• UV-Visible Absorbance (Abs)
• Fluorescence Intensity (FI)
• Time-Resolved Fluorescence (TRF)
• Fluorescence Polarization (FP)
• Glow Luminescence (Lumi)

Features

• Multiple Detection Modes
• Precision Optics and Detectors
• Patented AutoPMT Optimization System
• Patented PathCheck® Pathlength Measurement Technology
• Comprehensive Reader Validation
• Automating with Ease

General Photometric performance

Plate Formats

• 6, 12, 24, 48, 96, 384 wells

Light Source

• Xenon Flash Lamp (1 joule/flash)

Detectors

• 2 photomultiplier tubes

ShakerTime

• 0 to 999 seconds

Temperature Control

• 2 °C above ambient to 60 °C

Temperature Uniformity

• < 1°C at 37°C set point

Temperature Accuracy

• ±1°C at 37°C set point

Endpoint Reading

• All modes

Kinetic Reading

• All modes

Spectral Scanning

• All modes

Well Scanning

• Abs, FI, TRF, Lum

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

Features:

• G1364A Thermostatted Fraction Collector, preparative scale, can be created by additionally ordering and installing a G1330A Fraction Collector Thermostat

• For preparative scale applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
• Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
• Diverter valve: ~15
• Diverter valve to needle: ~110
• Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

Features:

• G1364C Fraction Collector, analytical scale, designed for flow rates below 10 ml / min. (can be modified for higher flow rates) and for the use with vials, test tubes of up to 75 mm height, well-plates and a 40-funnel tray connecting to external locations of any size 

• For analytical scale applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
• Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
• Diverter valve: ~15
• Diverter valve to needle: ~110
• Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

For Preparative Scale Applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
  • Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
  • Diverter valve: ~15
  • Diverter valve to needle: ~110
  • Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Features:

The detector is designed for highest optical performance, GLP compliance and easy maintenance. 

Specifications:

• Detection Type: 1024-element photodiode array
• Light Source: Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information.
• Data Rate: up to 80 Hz
• Wavelength Range: 190 – 950 nm
• Short Term: noise (ASTM) Single and Multi-Wavelength: < ± 0.7·10-5 AU at 254 and 750 nm
• Drift: < 0.9·10-3 AU/h at 254 nm 
• Linear Absorbance Range: > 2 AU (5 %) at 265 nm
• Wavelength Accuracy: ± 1 nm ,Self-calibration with deuterium lines, verification with holmium oxide filter
• Wavelength Bunching: 1 – 400 nm, Programmable in steps of 1 nm
• Slit Width: 1, 2, 4 , 8, 16 nm Programmable slit
• Diode Width: < 1 nm