Used Lab Equipment

Description:

Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results. The BD FACSCanto II can be configured with two or three lasers to detect up to eight colors. It features many innovations, including a true fixed alignment flow cell to minimize startup time and improve reproducibility. The optical system maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. These and other capabilities make the BD FACSCanto II ideal for today’s busy clinical lab, providing a high degree of automation and quality control. With optimal reproducibility, the BD FACSCanto II system reduces hands-on technician time and costs associated with repeat testing.

Specifications:

• Carryover: ≤0.1%
• Sample Injection: Direct into flow cell
• Max Particle Size: 50 μm
• Sample Flow Rate, Min: 10 μL/min
• Sample Flow Rate, Max: 120 μL/min
• Sample Acquisition Rate: 10,000 events/second, 6 compensated fluorescence parameters and 2 scatter parameters
• Sample Dead Volume: 30 μL
• Sheath Consumption: 1.10 L/h normal operation; <1 mL/h standby

Features:

• BD CSampler- 96 well plate and 24 tube rack automated sampler for BD Accuri C6 flow cytometer  
• Blue and red laser
• Two light scatter detectors
• Optical filters such as FITC, PE, PerCP and APC
• A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use
• Optional filters and the Selectable Laser Module expand the available fluorochrome combinations
• Compact system and portable weight (13.6 Kg)
• Intuitive interface of the software
• Measures: 11 x 14.75 x 16.5 inches (H x W x D) (27.9 x 37.5 x 41.9 cm)

Recent PM Guaranteed Installable:

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 3 laser, Blue(488) and Red (633) and Violet (405)
• 8 PMTs in 4-2-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• 405 nm Violet laser
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 8 PMTs in 4-2-2 configuration w/ side scatter

The Millipore Guava EasyCyte HT offers sample handling formats for both tubes and 96-well plates, direct sampling using patented microcapillary system, and a user-friendly, versatile software. EasyCyte instruments are also compatible with many fluorochromes and fluorescent reagents.

Specifications:

• Operating temperature: 16°–35°C (60°–95°F)
• Operating humidity: 10–90% relative humidity (non-condensing)
• Power: 100–240 VAC, 50/60 Hz, 120W
• Dimensions: 21 x 24 x 12 inches
• Weight: 71lb
• Detection: 5 colors including forward scatter
• Laser: blue 488 nm (50mW)
• Flow cell dimension: standard square capillary with ID of 100 µm
• Pump: 7 µL/min to 36 µL/min
• Sample flow rate: positive displacement
• Waste and cleaning vials: 30-mL glass vial with screw top
• Waste generation: typically <40 mL in 8 hours of continuous use
• Dead volume: 50 µL (for 96-well microplate); 75 µL (for 0.5-mL microcentrifuge tubes)
• Sample concentration: final particle concentration of 104 to 5 x 105 particles/ml
• Sample requirement: for accurate results (up to 2.5 x 106 beads/mL for the RapidQuant Assay) as few as 2,000 cells/test; typically 25,000-100,000 cells/test, depending on the assay
• Data management: Output data file formats - (1) binary data storage in FCS 3.0 format; (2) spreadsheet results file in CSV format

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• 3 PMTs in 4-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 1.0 μm
• SSC Resolution: 0.5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP

• Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter

Includes:

• Agilent G2938C Bioanalyzer
• Computer with Bioanalyzer Software
• Vortexer
• Priming Station

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

Areas of Application:

• On-chip flow cytometry
  • The easy way to acquire dual-color, cell-based fluorescence data
• RNA quality check with RIN
  • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
• DNA size and quantity
  • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
• SDS-PAGE replacement for protein analysis
  • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

Features:

• Faster than gels – analyze 10-12 samples in 30-40 minutes
• Easy to use – load the chip, press "start“, the instrument does the rest
• Ready-to-use assays and pre-packaged reagent kits
• Reproducible data, contamination-free hardware
• Quick and easy sample comparison – one-click overlay, scaling or zooming features
• Reproducible, complete and digital data
• Easy handling, storage and exchange of digital data
• Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
• Data is acquired and archived in digital format.

Single Laser Configuration

Includes Computer Loaded with Cytosoft Software

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

Features:

• Blue Laser
• Forward and Side Scatter
• Tube Sampling
• Automated 96 Well Micrplate Sampling
• Small Sample Size (<20 μL)
• Minimal waste (<50 mL per day)
• Detectors
  • Green
  • Yellow
  • Red

Fluidics Specifications:

• Flow Cell Dimension: Standard square capillary with ID of 100 µm
• Pump: Positive displacement
• Sheath Fluid: None
• Sample Flow Rate: 7µL/min to 72 µL/min
• Waste Generation: typically <40mL in 8 hours of continuous use
• Dead Volume: 50 µL (for 96-well microplate)
• Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
• Cell Size: 1-60 µm
• Typical # of Cells/Protocol: 1,000 – 10,000 cells/test

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes:

• Agilent G2938A Bioanalyzer
• Computer with Bioanalyzer Software
• Vortexer
• Priming Station

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

Areas of Application:

• On-chip flow cytometry
  • The easy way to acquire dual-color, cell-based fluorescence data
• RNA quality check with RIN
  • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
• DNA size and quantity
  • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
• SDS-PAGE replacement for protein analysis
  • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

Features:

• Faster than gels – analyze 10-12 samples in 30-40 minutes
• Easy to use – load the chip, press "start“, the instrument does the rest
• Ready-to-use assays and pre-packaged reagent kits
• Reproducible data, contamination-free hardware
• Quick and easy sample comparison – one-click overlay, scaling or zooming features
• Reproducible, complete and digital data
• Easy handling, storage and exchange of digital data
• Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
• Data is acquired and archived in digital format.

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software

• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• Laser
• 6 PMTs unit and side scatter

• All Necessary Cables
• Manuals

Optics Specifications:

• Lasers
  • Air-cooled:
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
• Blue Laser Dyes
  • FITC, PE, PerCP or PerCP-Cy™5.5,
  • PE-Cy7 (525, 575, 678 or 695, 785 nm)
• Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
• Detector Bands
  • Blue Laser: 530/30; 585/42; >670; 780/60 nm
  • Red Laser: 660/20; 780/60 nm
• Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF