We offer an assortment of used and refurbished Production Equipment ranging from fermenters, Bioreactors, Homogenizers, Chromotography Bioprocess Units to Production Scale Centrifuges. Some of our popular manufacturers include New Brunswick, Chemap, and Carr.
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The purification of biological therapeutics requires processes which usually employ two technologies used in combination: membrane separations and chromatography. Chromatography offers greater selectivity than membrane separations, and can purify much greater quantities of solution in one operation.
However, membrane separations technologies offer a number of other advantages including the fact that they are a fast and robust process step.
ÄKTAcrossflow covers three main filtration techniques:
The system supports both hollow fibers and flat sheet cassettes with sizes suitable for flow rates up to 300 ml/min for flat sheet cassettes and up to 600 ml/ min for hollow fibers.
The main application is method development and scale-up in process development laboratories.
The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.
Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.
The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.
Insect cell / Baculovirus
The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.